Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS19510 and BPHYT_RS19515 are separated by 53 nucleotidesBPHYT_RS19515 and BPHYT_RS19520 are separated by 147 nucleotidesBPHYT_RS19520 and BPHYT_RS19525 are separated by 94 nucleotidesBPHYT_RS19525 and BPHYT_RS19530 are separated by 105 nucleotides BPHYT_RS19510: BPHYT_RS19510 - adenylate cyclase, at 4,391,431 to 4,391,967 _RS19510 BPHYT_RS19515: BPHYT_RS19515 - AsnC family transcriptional regulator, at 4,392,021 to 4,392,512 _RS19515 BPHYT_RS19520: BPHYT_RS19520 - phenylalanine 4-monooxygenase, at 4,392,660 to 4,393,550 _RS19520 BPHYT_RS19525: BPHYT_RS19525 - pterin-4-alpha-carbinolamine dehydratase, at 4,393,645 to 4,393,935 _RS19525 BPHYT_RS19530: BPHYT_RS19530 - hypothetical protein, at 4,394,041 to 4,394,265 _RS19530
Group Condition BPHYT_RS19510 BPHYT_RS19515 BPHYT_RS19520 BPHYT_RS19525 BPHYT_RS19530
phage 36hr PHYTO7 MOI 1 -0.1 -0.8 +0.1 N.D. -0.4
phage PHYTO3 MOI 0.1 -0.1 -0.9 +0.1 N.D. -0.1
supernatant control Vogels_fungal_media; pH 5.5 -0.3 -0.7 +0.1 N.D. -0.0
supernatant control Vogels_fungal_media 0.1X -0.2 +0.2 -0.4 N.D. -0.6
nitrogen source L-Phenylalanine (N) -0.4 -0.3 -0.4 N.D. +0.1
supernatant control:fungal media R2A with Vogels_fungal_media 0.2X -0.3 +0.1 -0.3 N.D. -0.5
phage P. phyto PHYTO2 MOI 1 -0.3 -0.7 -0.0 N.D. +0.1
carbon source Malonic (C) +0.2 -0.5 -0.0 N.D. -0.6
carbon source D-Glucose (C) +0.2 -0.3 +0.1 N.D. -0.8
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.2 -0.5 +0.3 N.D. -0.4
carbon source L-Fucose (C) -0.1 -0.6 -0.3 N.D. +0.2
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.2 -0.5 +0.1 N.D. -0.6
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.2 -0.3 +0.4 N.D. -0.7
phage 24hr PHYTO4 MOI 10 -0.3 -0.7 +0.3 N.D. +0.3
carbon source Tween 20 (C) -0.3 +0.4 +0.0 N.D. -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.0 -0.2 -0.4 N.D. +0.5
temperature LB, 37C -0.3 -0.4 +0.0 N.D. +0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.5 -0.3 N.D. +0.2
phage 24hr PHYTO6 MOI 10 +0.1 -0.6 +0.2 N.D. +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 -0.7 +0.1 N.D. +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.2 +0.3 N.D. -0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 -0.4 +0.2 N.D. +0.6
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.1 +0.4 -0.4 N.D. +0.2
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.4 -0.1 +0.2 N.D. +0.8
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.1 -0.3 +0.3 N.D. +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.5 +0.7 N.D. +0.5
phage 36hr PHYTO3 MOI 1 -0.0 +0.1 +0.3 N.D. +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.9 +0.3 N.D. +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 +1.0 +0.3 N.D. -0.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.4 +0.5 -0.0 N.D. +0.6
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