Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS19490 and BPHYT_RS19495 are separated by 298 nucleotidesBPHYT_RS19495 and BPHYT_RS19500 are separated by 138 nucleotidesBPHYT_RS19500 and BPHYT_RS19505 are separated by 145 nucleotidesBPHYT_RS19505 and BPHYT_RS19510 are separated by 315 nucleotides BPHYT_RS19490: BPHYT_RS19490 - amino acid ABC transporter ATP-binding protein, at 4,385,603 to 4,386,382 _RS19490 BPHYT_RS19495: BPHYT_RS19495 - choline dehydrogenase, at 4,386,681 to 4,388,393 _RS19495 BPHYT_RS19500: BPHYT_RS19500 - methylmalonate-semialdehyde dehydrogenase, at 4,388,532 to 4,390,070 _RS19500 BPHYT_RS19505: BPHYT_RS19505 - LysR family transcriptional regulator, at 4,390,216 to 4,391,115 _RS19505 BPHYT_RS19510: BPHYT_RS19510 - adenylate cyclase, at 4,391,431 to 4,391,967 _RS19510
Group Condition BPHYT_RS19490 BPHYT_RS19495 BPHYT_RS19500 BPHYT_RS19505 BPHYT_RS19510
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.7 -0.6 -0.3 -0.4 -0.2
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.5 -0.3 -0.6 -0.4 -0.2
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.2 -0.4 -0.7 -0.3 -0.2
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -0.6 -0.1 -0.2 -0.4 -0.3
nitrogen source L-Alanine (N) -0.5 -0.0 -0.4 -0.6 -0.1
carbon source D-Glucose 20 mM (C) -0.6 -0.3 -0.0 -0.4 -0.3
nitrogen source L-Lysine (N) +0.0 -0.3 -0.5 -0.3 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.7 +0.1 -0.6 -0.1 -0.2
nitrogen source L-Serine (N) +0.2 -0.6 -0.3 -0.5 -0.2
phage 24hr PHYTO9 MOI 10 -0.4 -0.3 +0.1 -0.6 -0.2
carbon source Phenylacetic 5 mM (C) -1.2 -0.2 +0.3 +0.0 -0.2
carbon source Phenylacetic 5 mM (C) -0.9 +0.1 -0.2 -0.2 -0.1
nophagecontrol 36hr Phyto Lib only -0.6 -0.2 -0.4 +0.2 +0.1
phage 36hr PHYTO8 MOI 10 -0.9 -0.3 +0.2 +0.1 +0.1
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.4 +0.2 +0.5 -0.3 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.9 -0.1 -0.7 -0.1 -0.4
temperature LB, 37C -0.5 -0.1 +0.5 +0.1 -0.3
motility outer cut, LB soft agar motility assay -0.1 +0.0 -0.8 +0.6 -0.1
phage 36hr PHYTO6 MOI 0.1 -0.8 -0.1 +0.3 +0.4 -0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.6 +0.0 -0.6 -0.2 +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.7 +0.2 -0.4 -0.3 +0.0
phage 36hr PHYTO10 MOI 1 -0.6 +0.1 +0.6 +0.4 +0.0
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.8 +0.2 -0.4 -0.2 +0.1
carbon source Xylitol (C) +0.6 +0.1 -0.5 +0.4 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.7 -0.1 -0.3 +0.0 +0.4
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 +0.9 -0.3 -0.2 +0.1
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.9 -0.4 -0.2 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +0.5 +0.2 -0.2 +0.3
phage 24hr PHYTO3 MOI 10 -0.0 +0.5 +0.4 +0.8 +0.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.4 -0.0 -0.0 -0.1 +0.5
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