Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS19050 and BPHYT_RS19055 are separated by 163 nucleotidesBPHYT_RS19055 and BPHYT_RS19060 are separated by 131 nucleotidesBPHYT_RS19060 and BPHYT_RS19065 are separated by 140 nucleotidesBPHYT_RS19065 and BPHYT_RS19070 are separated by 402 nucleotides BPHYT_RS19050: BPHYT_RS19050 - hypothetical protein, at 4,276,364 to 4,276,675 _RS19050 BPHYT_RS19055: BPHYT_RS19055 - COF family hydrolase, at 4,276,839 to 4,277,660 _RS19055 BPHYT_RS19060: BPHYT_RS19060 - ATPase, at 4,277,792 to 4,278,673 _RS19060 BPHYT_RS19065: BPHYT_RS19065 - DNA-3-methyladenine glycosidase, at 4,278,814 to 4,279,410 _RS19065 BPHYT_RS19070: BPHYT_RS19070 - hypothetical protein, at 4,279,813 to 4,280,238 _RS19070
Group Condition BPHYT_RS19050 BPHYT_RS19055 BPHYT_RS19060 BPHYT_RS19065 BPHYT_RS19070
carbon source D-Glucosamine Hydrochloride (C) +0.1 -2.3 -0.1 -0.1 +0.1
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.5 -0.0 -0.3 -0.5 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.0 +0.3 -0.8 +0.2 -1.0
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.1 -0.2 -0.6 +0.1 -0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.3 -0.1 -0.7 -0.1 -0.4
nitrogen source L-Proline (N) +0.2 -0.3 -0.2 -0.1 -0.7
supernatant control Vogels_fungal_media; pH 5.5 +0.1 -0.1 -0.4 -0.3 -0.3
nitrogen source L-Serine (N) -0.4 -0.2 -0.2 -0.3 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.2 +0.4 -0.5 -0.2 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.2 +0.1 +0.3 -0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.5 +0.1 -0.4 -0.4 -0.2
phage 36hr PHYTO11 MOI 1 +0.3 +0.0 -0.1 -0.5 +0.4
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.3 -0.2 +0.1 +0.1 +0.6
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.6 -0.1 +0.3 -0.3 -0.0
phage 24hr PHYTO9 MOI 0.1 -0.1 +0.2 +0.4 -0.4 +0.4
phage 36hr PHYTO6 MOI 10 +0.1 +0.2 +0.2 -0.4 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.7 +0.3 -0.4 +0.1 -0.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 -0.3 +0.2 +0.2 +0.5
phage 36hr PHYTO2 MOI 1 +0.0 +0.3 -0.3 +0.0 +0.7
phage 24hr PHYTO10 MOI 1 +0.2 +0.2 +0.2 -0.3 +0.4
phage 24hr PHYTO4 MOI 10 +0.1 +0.2 +0.1 -0.2 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 +0.2 +0.3 -0.2 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.1 +0.5 +0.4 +0.0 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.0 +0.4 +0.4 +0.2
phage 24hr PHYTO3 MOI 10 +0.3 +0.3 +0.5 -0.3 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.6 -0.0 +0.3 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.3 +0.4 -0.1 +0.2 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days +0.3 +0.3 +0.3 +0.3 +0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.1 +0.4 +0.6 +0.2 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +0.6 +0.5 -0.2 +0.1
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