Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS18765 and BPHYT_RS18770 are separated by 216 nucleotidesBPHYT_RS18770 and BPHYT_RS18775 are separated by 235 nucleotidesBPHYT_RS18775 and BPHYT_RS18780 overlap by 11 nucleotidesBPHYT_RS18780 and BPHYT_RS18785 overlap by 8 nucleotides BPHYT_RS18765: BPHYT_RS18765 - flagellar biosynthesis protein FliS, at 4,219,874 to 4,220,308 _RS18765 BPHYT_RS18770: BPHYT_RS18770 - flagellar hook-basal body protein FliE, at 4,220,525 to 4,220,860 _RS18770 BPHYT_RS18775: BPHYT_RS18775 - flagellar M-ring protein FliF, at 4,221,096 to 4,222,907 _RS18775 BPHYT_RS18780: BPHYT_RS18780 - flagellar motor switch protein FliG, at 4,222,897 to 4,223,892 _RS18780 BPHYT_RS18785: BPHYT_RS18785 - flagellar assembly protein FliH, at 4,223,885 to 4,224,562 _RS18785
Group Condition BPHYT_RS18765 BPHYT_RS18770 BPHYT_RS18775 BPHYT_RS18780 BPHYT_RS18785
motility outer cut, LB soft agar motility assay -3.2 N.D. -3.8 -3.6 -5.7
motility outer cut, LB soft agar motility assay -2.7 N.D. -4.4 -2.2 -5.7
motility inner cut, LB soft agar motility assay -3.4 N.D. -2.9 -1.5 -3.8
motility inner cut, LB soft agar motility assay -3.1 N.D. -2.6 -1.4 -2.3
motility inner cut, LB soft agar motility assay -2.9 N.D. -2.9 -1.4 -1.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.6 N.D. -2.3 -2.3 -2.7
motility outer cut, LB soft agar motility assay -2.6 N.D. -2.1 -1.3 -2.7
stress Bacitracin 0.25 mg/ml -0.9 N.D. -2.2 -2.1 -3.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 N.D. -1.8 -1.6 -1.7
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -2.0 N.D. -1.3 -1.0 -1.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.2 N.D. -1.4 -1.1 -2.1
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -2.6 N.D. -0.6 -0.4 -2.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.9 N.D. -1.4 -1.4 -1.9
vitamin No vitamins t2 -1.4 N.D. +1.2 +0.7 +1.1
carbon source D-Ribose (C) -1.6 N.D. +1.3 +1.1 +1.5
nitrogen source D-Alanine (N) -1.3 N.D. +1.3 +1.4 +1.1
vitamin With vitamins t2 -1.0 N.D. +1.3 +0.9 +1.4
nitrogen source L-Phenylalanine (N) -0.8 N.D. +1.2 +1.3 +1.1
carbon source L-Phenylalanine (C) -0.8 N.D. +1.2 +1.2 +1.2
carbon source D-Sorbitol (C) -0.8 N.D. +1.4 +1.3 +1.1
carbon source L-Asparagine (C) -0.7 N.D. +1.4 +1.2 +1.3
stress Paraquat dichloride 0.064 mg/ml -0.8 N.D. +1.5 +1.2 +1.3
nitrogen source L-Citrulline (N) -0.9 N.D. +1.3 +1.2 +1.7
carbon source Xylitol (C) -0.6 N.D. +1.2 +1.3 +1.7
nitrogen source Carnitine Hydrochloride (N) -0.6 N.D. +1.3 +1.2 +1.8
carbon source Malonic (C) -0.4 N.D. +1.4 +1.1 +1.8
nitrogen source L-Isoleucine (N) -0.6 N.D. +1.4 +1.3 +2.0
carbon source Malonic (C) -0.3 N.D. +1.4 +1.4 +2.0
carbon source D-Xylose (C) -0.1 N.D. +1.8 +1.8 +1.9
carbon source Gly-Glu (C) +0.0 N.D. +2.3 +2.2 +2.2
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