Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS18325 and BPHYT_RS18330 are separated by 265 nucleotidesBPHYT_RS18330 and BPHYT_RS18335 are separated by 216 nucleotidesBPHYT_RS18335 and BPHYT_RS18340 are separated by 184 nucleotidesBPHYT_RS18340 and BPHYT_RS18345 are separated by 494 nucleotides BPHYT_RS18325: BPHYT_RS18325 - LuxR family transcriptional regulator, at 4,123,030 to 4,123,719 _RS18325 BPHYT_RS18330: BPHYT_RS18330 - phosphatidylethanolamine-binding protein, at 4,123,985 to 4,124,587 _RS18330 BPHYT_RS18335: BPHYT_RS18335 - NADPH-dependent FMN reductase, at 4,124,804 to 4,125,376 _RS18335 BPHYT_RS18340: BPHYT_RS18340 - n-acetyl-gamma-glutamyl-phosphate reductase, at 4,125,561 to 4,126,508 _RS18340 BPHYT_RS18345: BPHYT_RS18345 - hypothetical protein, at 4,127,003 to 4,128,433 _RS18345
Group Condition BPHYT_RS18325 BPHYT_RS18330 BPHYT_RS18335 BPHYT_RS18340 BPHYT_RS18345
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.1 -0.2 -0.1 -4.7 -0.1
carbon source D-Xylose (C) -0.2 +0.1 -0.2 -4.5 +0.0
nitrogen source L-Isoleucine (N) -0.1 -0.1 -0.2 -3.6 +0.0
carbon source Phenylacetic 5 mM (C) +0.3 -0.6 +0.2 -3.8 -0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.1 -0.1 +0.0 -3.6 -0.2
carbon source Tween 20 (C) -0.4 -0.2 -0.3 -2.6 -0.0
carbon source L-Lactate (C) -0.2 -0.2 +0.0 -3.2 +0.1
supernatant control Vogels_fungal_media; pH 5.5 -0.0 -0.3 -0.6 -2.4 -0.2
nitrogen source L-Asparagine (N) +0.1 +0.1 -0.0 -3.8 +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.3 +0.4 -0.3 -3.4 -0.3
nitrogen source L-Phenylalanine (N) -0.1 +0.0 -0.1 -3.1 +0.0
nitrogen source Uridine (N) +0.2 +0.2 -0.0 -3.4 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.2 -0.3 -0.2 -2.5 -0.1
nitrogen source L-Serine (N) +0.1 -0.1 +0.3 -3.5 +0.0
nitrogen source NAG (N) +0.3 +0.2 -0.0 -3.3 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.1 +0.2 -0.5 -2.6 +0.0
nitrogen source L-Lysine (N) +0.3 +0.5 -0.1 -3.3 +0.1
nitrogen source Ammonium chloride (N) +0.3 -0.0 +0.0 -2.8 -0.0
nitrogen source L-Alanine (N) +0.3 +0.1 -0.1 -3.0 +0.1
carbon source L-Alanine (C) +0.0 +0.2 +0.1 -2.7 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.1 +0.3 -3.1 +0.1
temperature LB, 23C -0.2 +0.2 -0.2 -2.4 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.2 +0.5 -3.2 +0.1
carbon source 2-Deoxy-D-ribonic lithium salt (C) +0.5 -0.8 -0.2 -1.5 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.6 +0.1 -2.2 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.2 -0.0 +0.8 -2.2 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +1.1 +0.4 -2.1 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.0 +0.5 -0.1 -2.0 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +1.0 +0.5 -2.4 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 +0.4 +0.7 +1.1 +0.6
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