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Protein
Homologs
Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS18325 and BPHYT_RS18330 are separated by 265 nucleotides
BPHYT_RS18330 and BPHYT_RS18335 are separated by 216 nucleotides
BPHYT_RS18335 and BPHYT_RS18340 are separated by 184 nucleotides
BPHYT_RS18340 and BPHYT_RS18345 are separated by 494 nucleotides
BPHYT_RS18325: BPHYT_RS18325 - LuxR family transcriptional regulator, at 4,123,030 to 4,123,719
_RS18325
BPHYT_RS18330: BPHYT_RS18330 - phosphatidylethanolamine-binding protein, at 4,123,985 to 4,124,587
_RS18330
BPHYT_RS18335: BPHYT_RS18335 - NADPH-dependent FMN reductase, at 4,124,804 to 4,125,376
_RS18335
BPHYT_RS18340: BPHYT_RS18340 - n-acetyl-gamma-glutamyl-phosphate reductase, at 4,125,561 to 4,126,508
_RS18340
BPHYT_RS18345: BPHYT_RS18345 - hypothetical protein, at 4,127,003 to 4,128,433
_RS18345
Group
Condition
BPHYT
_RS18325
BPHYT
_RS18330
BPHYT
_RS18335
BPHYT
_RS18340
BPHYT
_RS18345
replicable vector efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
-0.1
-0.2
-0.1
-4.7
-0.1
carbon source
D-Xylose (C)
-0.2
+0.1
-0.2
-4.5
+0.0
nitrogen source
L-Isoleucine (N)
-0.1
-0.1
-0.2
-3.6
+0.0
carbon source
Phenylacetic 5 mM (C)
+0.3
-0.6
+0.2
-3.8
-0.0
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
+0.1
-0.1
+0.0
-3.6
-0.2
carbon source
Tween 20 (C)
-0.4
-0.2
-0.3
-2.6
-0.0
carbon source
L-Lactate (C)
-0.2
-0.2
+0.0
-3.2
+0.1
supernatant control
Vogels_fungal_media; pH 5.5
-0.0
-0.3
-0.6
-2.4
-0.2
nitrogen source
L-Asparagine (N)
+0.1
+0.1
-0.0
-3.8
+0.0
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB
+0.3
+0.4
-0.3
-3.4
-0.3
nitrogen source
L-Phenylalanine (N)
-0.1
+0.0
-0.1
-3.1
+0.0
nitrogen source
Uridine (N)
+0.2
+0.2
-0.0
-3.4
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.2
-0.3
-0.2
-2.5
-0.1
nitrogen source
L-Serine (N)
+0.1
-0.1
+0.3
-3.5
+0.0
nitrogen source
NAG (N)
+0.3
+0.2
-0.0
-3.3
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
+0.1
+0.2
-0.5
-2.6
+0.0
nitrogen source
L-Lysine (N)
+0.3
+0.5
-0.1
-3.3
+0.1
nitrogen source
Ammonium chloride (N)
+0.3
-0.0
+0.0
-2.8
-0.0
nitrogen source
L-Alanine (N)
+0.3
+0.1
-0.1
-3.0
+0.1
carbon source
L-Alanine (C)
+0.0
+0.2
+0.1
-2.7
-0.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.2
+0.1
+0.3
-3.1
+0.1
temperature
LB, 23C
-0.2
+0.2
-0.2
-2.4
+0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.1
+0.2
+0.5
-3.2
+0.1
carbon source
2-Deoxy-D-ribonic lithium salt (C)
+0.5
-0.8
-0.2
-1.5
+0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.1
+0.6
+0.1
-2.2
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.2
-0.0
+0.8
-2.2
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.1
+1.1
+0.4
-2.1
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+1.0
+0.5
-0.1
-2.0
+0.3
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.5
+1.0
+0.5
-2.4
+0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.5
+0.4
+0.7
+1.1
+0.6
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