Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS18235 and BPHYT_RS18240 are separated by 499 nucleotidesBPHYT_RS18240 and BPHYT_RS18245 are separated by 91 nucleotidesBPHYT_RS18245 and BPHYT_RS18250 are separated by 215 nucleotidesBPHYT_RS18250 and BPHYT_RS18255 are separated by 38 nucleotides BPHYT_RS18235: BPHYT_RS18235 - 16S ribosomal RNA, at 4,100,124 to 4,101,718 _RS18235 BPHYT_RS18240: BPHYT_RS18240 - GDSL family lipase, at 4,102,218 to 4,103,486 _RS18240 BPHYT_RS18245: BPHYT_RS18245 - hypothetical protein, at 4,103,578 to 4,105,200 _RS18245 BPHYT_RS18250: BPHYT_RS18250 - phenylacetate-CoA oxygenase subunit PaaA, at 4,105,416 to 4,106,414 _RS18250 BPHYT_RS18255: BPHYT_RS18255 - phenylacetate-CoA oxygenase subunit PaaB, at 4,106,453 to 4,106,737 _RS18255
Group Condition BPHYT_RS18235 BPHYT_RS18240 BPHYT_RS18245 BPHYT_RS18250 BPHYT_RS18255
carbon source L-Phenylalanine (C) N.D. +0.2 -0.0 -6.0 -5.5
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. +0.1 -0.0 -2.6 -4.6
nitrogen source L-Phenylalanine (N) N.D. +0.2 -0.0 -3.0 -4.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -0.2 -0.2 -0.7 -5.6
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. +0.0 -0.1 -1.9 -3.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.1 -0.1 -1.3 -4.0
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. -0.0 -0.0 -2.2 -2.7
carbon source Phenylacetic 5 mM (C) N.D. +0.1 +0.2 -2.0 -3.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. +0.2 -0.2 -1.6 -3.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.0 +0.1 -2.2 -2.1
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.1 +0.0 -1.9 -2.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.0 -0.0 -1.0 -2.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 +0.1 -1.0 -2.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.0 +0.0 -1.2 -2.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.1 +0.1 -1.3 -2.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.3 -0.1 -1.7 -2.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.1 -0.0 -1.2 -2.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.0 +0.1 -1.5 -2.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.2 +0.2 -2.1 -1.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.2 -0.2 -1.2 -2.0
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. +0.2 +0.0 -1.2 -2.4
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. +0.2 +0.1 -0.8 -2.8
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.2 +0.1 -1.5 -2.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 +0.0 -1.2 -2.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.0 +0.1 -1.4 -2.0
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 +0.1 -1.4 -1.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 -0.0 -0.8 -2.4
tn7 efficiency Outgrowth after Tn7 transformation N.D. +0.1 +0.1 -0.5 -2.7
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.0 +0.2 -1.6 -1.5
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.2 +0.1 -1.1 -1.9
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