Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS18130 and BPHYT_RS18135 are separated by 134 nucleotidesBPHYT_RS18135 and BPHYT_RS18140 are separated by 178 nucleotidesBPHYT_RS18140 and BPHYT_RS18145 are separated by 222 nucleotidesBPHYT_RS18145 and BPHYT_RS18150 are separated by 218 nucleotides BPHYT_RS18130: BPHYT_RS18130 - elongation factor G, at 4,076,716 to 4,078,818 _RS18130 BPHYT_RS18135: BPHYT_RS18135 - 30S ribosomal protein S7, at 4,078,953 to 4,079,423 _RS18135 BPHYT_RS18140: BPHYT_RS18140 - 30S ribosomal protein S12, at 4,079,602 to 4,079,982 _RS18140 BPHYT_RS18145: BPHYT_RS18145 - ATP-dependent DNA helicase RecQ, at 4,080,205 to 4,082,052 _RS18145 BPHYT_RS18150: BPHYT_RS18150 - DNA-directed RNA polymerase subunit beta', at 4,082,271 to 4,086,509 _RS18150
Group Condition BPHYT_RS18130 BPHYT_RS18135 BPHYT_RS18140 BPHYT_RS18145 BPHYT_RS18150
carbon source Tween 20 (C) N.D. N.D. N.D. -0.3 N.D.
stress Cobalt chloride 0.25 mM N.D. N.D. N.D. -0.3 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. N.D. N.D. -0.3 N.D.
carbon source Sebacic (C) N.D. N.D. N.D. +0.3 N.D.
nitrogen source L-Phenylalanine (N) N.D. N.D. N.D. +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. N.D. +0.3 N.D.
phage 24hr PHYTO4 MOI 0.1 N.D. N.D. N.D. +0.3 N.D.
phage P. phyto PHYTO2 MOI 1 N.D. N.D. N.D. +0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. +0.3 N.D.
carbon source D-Glucose (C) N.D. N.D. N.D. +0.3 N.D.
stress Paraquat dichloride 0.032 mg/ml N.D. N.D. N.D. +0.3 N.D.
phage 36hr PHYTO4 MOI 1 N.D. N.D. N.D. +0.3 N.D.
nitrogen source L-Serine (N) N.D. N.D. N.D. +0.3 N.D.
phage 24hr PHYTO9 MOI 0.1 N.D. N.D. N.D. +0.3 N.D.
phage 36hr PHYTO11 MOI 0.1 N.D. N.D. N.D. +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. +0.3 N.D.
phage 36hr PHYTO MOI 0.1 N.D. N.D. N.D. +0.3 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. N.D. +0.3 N.D.
nophagecontrol 36hr Phyto Lib Only N.D. N.D. N.D. +0.3 N.D.
phage 36hr PHYTO10 MOI 10 N.D. N.D. N.D. +0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. N.D. +0.4 N.D.
phage 24hr PHYTO10 MOI 10 N.D. N.D. N.D. +0.4 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. +0.4 N.D.
phage 24hr PHYTO11 MOI 1 N.D. N.D. N.D. +0.4 N.D.
tn7 efficiency Outgrowth after Tn7 transformation N.D. N.D. N.D. +0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. N.D. +0.4 N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. N.D. N.D. +0.4 N.D.
phage 36hr PHYTO6 MOI 0.1 N.D. N.D. N.D. +0.5 N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. N.D. N.D. +0.5 N.D.
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. N.D. N.D. +0.5 N.D.
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