Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS17420 and BPHYT_RS17425 are separated by 154 nucleotidesBPHYT_RS17425 and BPHYT_RS17430 are separated by 94 nucleotidesBPHYT_RS17430 and BPHYT_RS17435 are separated by 127 nucleotidesBPHYT_RS17435 and BPHYT_RS17440 are separated by 102 nucleotides BPHYT_RS17420: BPHYT_RS17420 - FMN-dependent NADH-azoreductase, at 3,946,190 to 3,946,786 _RS17420 BPHYT_RS17425: BPHYT_RS17425 - peptidase M61, at 3,946,941 to 3,948,731 _RS17425 BPHYT_RS17430: BPHYT_RS17430 - thiol:disulfide interchange protein, at 3,948,826 to 3,949,551 _RS17430 BPHYT_RS17435: BPHYT_RS17435 - ubiquinone biosynthesis hydroxylase UbiH, at 3,949,679 to 3,950,851 _RS17435 BPHYT_RS17440: BPHYT_RS17440 - hypothetical protein, at 3,950,954 to 3,951,319 _RS17440
Group Condition BPHYT_RS17420 BPHYT_RS17425 BPHYT_RS17430 BPHYT_RS17435 BPHYT_RS17440
phage 36hr PHYTO6 MOI 0.1 -0.3 +0.0 -0.8 N.D. -1.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.3 +0.0 +0.0 N.D. -2.5
phage 36hr PHYTO4 MOI 1 -0.3 -0.1 -1.2 N.D. -0.6
phage 24hr PHYTO3 MOI 0.1 -0.0 -0.0 -1.2 N.D. -0.5
phage 24hr PHYTO10 MOI 10 -0.1 -0.1 -0.9 N.D. -0.7
phage 36hr PHYTO2 MOI 1 -0.2 +0.1 -0.8 N.D. -0.8
phage 24hr PHYTO1 MOI 10 -0.1 +0.1 -1.0 N.D. -0.7
phage 36hr PHYTO11 MOI 1 +0.0 -0.0 -1.7 N.D. +0.0
phage 36hr PHYTO7 MOI 0.1 -0.0 +0.1 -0.6 N.D. -1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.1 -1.1 N.D. -0.1
no phage control No phage control -0.0 +0.1 -0.6 N.D. -0.9
phage 24hr PHYTO2 MOI 1 -0.2 -0.1 -1.2 N.D. +0.2
nitrogen source L-Lysine (N) +0.3 +0.3 -1.1 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.5 -0.0 +0.4 N.D. -1.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.3 -0.1 -0.4 N.D. -0.7
phage 36hr PHYTO10 MOI 10 +0.4 -0.2 -0.4 N.D. -0.7
phage 24hr PHYTO6 MOI 1 +0.2 +0.0 -1.3 N.D. +0.2
nophagecontrol 36hr Phyto Lib only +0.2 +0.3 -1.2 N.D. -0.1
supernatant control Vogels_fungal_media; pH 5.5 +0.1 +0.1 +0.3 N.D. -1.4
phage P. phyto corn MOI 1 +0.1 -0.2 -0.9 N.D. +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.0 -1.2 N.D. +0.9
carbon source D-Glucose (C) -0.4 +0.1 -0.7 N.D. +0.4
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.1 -0.0 -1.2 N.D. +0.8
nophagecontrol 24hr Phyto Lib Only +0.2 -0.0 -1.0 N.D. +0.5
phage 24hr PHYTO11 MOI 1 +0.2 -0.1 -0.9 N.D. +0.7
phage 24hr PHYTO8 MOI 1 +0.1 -0.2 -1.0 N.D. +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.6 +0.3 -0.3 N.D. -0.6
nophagecontrol 24hr Phyto Lib only +0.4 -0.1 -0.9 N.D. +0.6
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.1 -0.2 -0.1 N.D. +1.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.2 -0.2 +1.9 N.D. +0.5
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