Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS17410 and BPHYT_RS17415 are separated by 84 nucleotidesBPHYT_RS17415 and BPHYT_RS17420 are separated by 128 nucleotidesBPHYT_RS17420 and BPHYT_RS17425 are separated by 154 nucleotidesBPHYT_RS17425 and BPHYT_RS17430 are separated by 94 nucleotides BPHYT_RS17410: BPHYT_RS17410 - adenylate cyclase, at 3,944,505 to 3,945,131 _RS17410 BPHYT_RS17415: BPHYT_RS17415 - uracil-DNA glycosylase, at 3,945,216 to 3,946,061 _RS17415 BPHYT_RS17420: BPHYT_RS17420 - FMN-dependent NADH-azoreductase, at 3,946,190 to 3,946,786 _RS17420 BPHYT_RS17425: BPHYT_RS17425 - peptidase M61, at 3,946,941 to 3,948,731 _RS17425 BPHYT_RS17430: BPHYT_RS17430 - thiol:disulfide interchange protein, at 3,948,826 to 3,949,551 _RS17430
Group Condition BPHYT_RS17410 BPHYT_RS17415 BPHYT_RS17420 BPHYT_RS17425 BPHYT_RS17430
phage 36hr PHYTO11 MOI 1 -0.1 N.D. +0.0 -0.0 -1.7
phage 36hr PHYTO4 MOI 1 +0.2 N.D. -0.3 -0.1 -1.2
phage 24hr PHYTO2 MOI 1 +0.2 N.D. -0.2 -0.1 -1.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 N.D. -0.2 -0.0 -1.2
phage P. phyto corn MOI 1 -0.3 N.D. +0.1 -0.2 -0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 N.D. -0.3 +0.1 -1.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 N.D. +0.2 -0.3 -0.4
phage 24hr PHYTO6 MOI 1 -0.0 N.D. +0.2 +0.0 -1.3
phage 24hr PHYTO9 MOI 0.1 +0.4 N.D. -0.0 -0.2 -1.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.4 N.D. +0.1 -0.0 -1.2
nophagecontrol 36hr Phyto Lib only +0.4 N.D. -0.4 -0.0 -0.6
phage 24hr PHYTO8 MOI 1 +0.4 N.D. +0.1 -0.2 -1.0
nophagecontrol 36hr Phyto Lib only +0.2 N.D. +0.2 +0.3 -1.2
phage 36hr PHYTO2 MOI 1 +0.4 N.D. -0.2 +0.1 -0.8
nophagecontrol 24hr Phyto Lib only +0.3 N.D. +0.4 -0.1 -0.9
nitrogen source L-Lysine (N) +0.4 N.D. +0.3 +0.3 -1.1
phage 36hr PHYTO8 MOI 10 +0.4 N.D. +0.2 +0.2 -0.8
phage P. phyto PHYTO2 MOI 1 +0.5 N.D. +0.3 -0.1 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.7 N.D. +0.1 +0.2 -0.9
motility outer cut, LB soft agar motility assay +0.5 N.D. +0.4 +0.2 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.4 N.D. +0.4 +0.2 -0.6
phage 36hr PHYTO10 MOI 10 +0.7 N.D. +0.4 -0.2 -0.4
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -0.3 N.D. -0.0 -0.0 +1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.4 N.D. +0.6 +0.3 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.7 N.D. +0.4 +0.3 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.5 N.D. +0.5 -0.0 +0.4
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.5 N.D. +0.2 -0.2 +1.9
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.3 N.D. +0.5 +0.5 +0.3
carbon source D-Xylose (C) +0.6 N.D. +0.2 +0.2 +0.6
stress Lomefloxacin 0.001 mM +0.5 N.D. +0.4 +0.5 +0.4
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