Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS17230 and BPHYT_RS17235 overlap by 4 nucleotidesBPHYT_RS17235 and BPHYT_RS17240 overlap by 4 nucleotidesBPHYT_RS17240 and BPHYT_RS17245 overlap by 4 nucleotidesBPHYT_RS17245 and BPHYT_RS17250 are separated by 26 nucleotides BPHYT_RS17230: BPHYT_RS17230 - UDP-N-acetylmuramate--L-alanine ligase, at 3,902,622 to 3,904,025 _RS17230 BPHYT_RS17235: BPHYT_RS17235 - UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase, at 3,904,022 to 3,905,140 _RS17235 BPHYT_RS17240: BPHYT_RS17240 - cell division protein FtsW, at 3,905,137 to 3,906,414 _RS17240 BPHYT_RS17245: BPHYT_RS17245 - UDP-N-acetylmuramoylalanine--D-glutamate ligase, at 3,906,411 to 3,907,925 _RS17245 BPHYT_RS17250: BPHYT_RS17250 - phospho-N-acetylmuramoyl-pentapeptide- transferase, at 3,907,952 to 3,909,121 _RS17250
Group Condition BPHYT_RS17230 BPHYT_RS17235 BPHYT_RS17240 BPHYT_RS17245 BPHYT_RS17250
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -4.5 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -4.3 N.D. N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. N.D. -4.1 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -3.2 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -3.0 N.D. N.D.
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. N.D. -3.0 N.D. N.D.
carbon source Malonic (C) N.D. N.D. -2.9 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -2.8 N.D. N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. N.D. -2.8 N.D. N.D.
motility outer cut, LB soft agar motility assay N.D. N.D. -2.7 N.D. N.D.
nitrogen source L-Lysine (N) N.D. N.D. -2.7 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -2.6 N.D. N.D.
phage P. phyto PHYTO2 MOI 1 N.D. N.D. -2.5 N.D. N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. N.D. -2.5 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -2.5 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -2.4 N.D. N.D.
temperature LB 37C N.D. N.D. -2.2 N.D. N.D.
carbon source succinate (C) N.D. N.D. -2.2 N.D. N.D.
phage P. phyto PHYTO2 MOI 1 N.D. N.D. -2.2 N.D. N.D.
stress A22 0.005 mg/ml N.D. N.D. -2.2 N.D. N.D.
lb_dap plate control LB_DAP agar plate, outgrowth in LB N.D. N.D. -2.1 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. -2.1 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. -2.0 N.D. N.D.
phage P. phyto PHYTO2 MOI 0.1 N.D. N.D. -2.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. +2.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. +2.2 N.D. N.D.
phage 36hr PHYTO11 MOI 0.1 N.D. N.D. +2.2 N.D. N.D.
phage 36hr PHYTO11 MOI 1 N.D. N.D. +2.2 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. +2.8 N.D. N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. N.D. +5.8 N.D. N.D.
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