Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS17205 and BPHYT_RS17210 are separated by 256 nucleotidesBPHYT_RS17210 and BPHYT_RS17215 are separated by 151 nucleotidesBPHYT_RS17215 and BPHYT_RS17220 are separated by 27 nucleotidesBPHYT_RS17220 and BPHYT_RS17225 are separated by 48 nucleotides BPHYT_RS17205: BPHYT_RS17205 - peroxiredoxin, at 3,897,510 to 3,898,013 _RS17205 BPHYT_RS17210: BPHYT_RS17210 - cell division protein FtsZ, at 3,898,270 to 3,899,466 _RS17210 BPHYT_RS17215: BPHYT_RS17215 - cell division protein FtsA, at 3,899,618 to 3,900,850 _RS17215 BPHYT_RS17220: BPHYT_RS17220 - cell division protein FtsQ, at 3,900,878 to 3,901,630 _RS17220 BPHYT_RS17225: BPHYT_RS17225 - D-alanine--D-alanine ligase, at 3,901,679 to 3,902,620 _RS17225
Group Condition BPHYT_RS17205 BPHYT_RS17210 BPHYT_RS17215 BPHYT_RS17220 BPHYT_RS17225
phage 24hr PHYTO6 MOI 10 -1.4 N.D. N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 N.D. N.D. N.D. N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -1.0 N.D. N.D. N.D. N.D.
phage P. phyto PHYTO2 MOI 1 -1.0 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.8 N.D. N.D. N.D. N.D.
phage 24hr PHYTO3 MOI 10 -0.8 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 N.D. N.D. N.D. N.D.
nitrogen source Carnitine Hydrochloride (N) -0.8 N.D. N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.8 N.D. N.D. N.D. N.D.
carbon source Suberic (C) -0.7 N.D. N.D. N.D. N.D.
stress Paraquat dichloride 0.032 mg/ml -0.7 N.D. N.D. N.D. N.D.
carbon source D-Galacturonic Acid (C) -0.7 N.D. N.D. N.D. N.D.
carbon source Protocatechuic Acid (C) -0.7 N.D. N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.7 N.D. N.D. N.D. N.D.
pH LB, pH4 -0.7 N.D. N.D. N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.7 N.D. N.D. N.D. N.D.
carbon source succinate (C) -0.7 N.D. N.D. N.D. N.D.
phage 24hr PHYTO11 MOI 1 -0.7 N.D. N.D. N.D. N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.7 N.D. N.D. N.D. N.D.
tn7 efficiency Outgrowth after Tn7 transformation +0.7 N.D. N.D. N.D. N.D.
phage PHYTO3 MOI 0.1 +0.7 N.D. N.D. N.D. N.D.
phage 24hr PHYTO8 MOI 1 +0.8 N.D. N.D. N.D. N.D.
nutrient casaminos +0.8 N.D. N.D. N.D. N.D.
carbon source Phenylacetic 5 mM (C) +0.9 N.D. N.D. N.D. N.D.
carbon source Sebacic (C) +0.9 N.D. N.D. N.D. N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.9 N.D. N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.0 N.D. N.D. N.D. N.D.
carbon source D-Glucose 20 mM (C) +1.2 N.D. N.D. N.D. N.D.
carbon source Phenylacetic 5 mM (C) +1.3 N.D. N.D. N.D. N.D.
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +1.4 N.D. N.D. N.D. N.D.
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