Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS17005 and BPHYT_RS17010 are separated by 67 nucleotidesBPHYT_RS17010 and BPHYT_RS17015 are separated by 130 nucleotidesBPHYT_RS17015 and BPHYT_RS17020 are separated by 3 nucleotidesBPHYT_RS17020 and BPHYT_RS17025 are separated by 48 nucleotides BPHYT_RS17005: BPHYT_RS17005 - 3-dehydroquinate dehydratase, at 3,856,097 to 3,856,552 _RS17005 BPHYT_RS17010: BPHYT_RS17010 - acetyl-CoA carboxylase, at 3,856,620 to 3,857,090 _RS17010 BPHYT_RS17015: BPHYT_RS17015 - acetyl-CoA carboxylase biotin carboxylase subunit, at 3,857,221 to 3,858,588 _RS17015 BPHYT_RS17020: BPHYT_RS17020 - ribosomal protein L11 methyltransferase, at 3,858,592 to 3,859,494 _RS17020 BPHYT_RS17025: BPHYT_RS17025 - hypothetical protein, at 3,859,543 to 3,860,943 _RS17025
Group Condition BPHYT_RS17005 BPHYT_RS17010 BPHYT_RS17015 BPHYT_RS17020 BPHYT_RS17025
nophagecontrol 24hr Phyto Lib Only N.D. N.D. N.D. -0.5 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. -0.7 -0.3
phage 24hr PHYTO4 MOI 0.1 N.D. N.D. N.D. -0.8 -0.2
phage 24hr PHYTO9 MOI 0.1 N.D. N.D. N.D. -0.3 -0.7
phage 36hr PHYTO11 MOI 1 N.D. N.D. N.D. -0.6 -0.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. N.D. -0.5 -0.4
phage PHYTO3 MOI 0.1 N.D. N.D. N.D. -0.4 -0.5
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. -0.6 -0.2
nophagecontrol 36hr Phyto Lib only N.D. N.D. N.D. -0.5 -0.3
phage 24hr PHYTO8 MOI 1 N.D. N.D. N.D. -0.2 -0.6
phage 36hr PHYTO3 MOI 1 N.D. N.D. N.D. -0.6 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. -0.6 -0.2
phage 36hr PHYTO4 MOI 1 N.D. N.D. N.D. -0.5 -0.3
phage 36hr PHYTO10 MOI 10 N.D. N.D. N.D. -0.4 -0.4
phage 24hr PHYTO10 MOI 10 N.D. N.D. N.D. -0.3 -0.4
phage 24hr PHYTO3 MOI 10 N.D. N.D. N.D. -0.3 -0.4
phage 24hr PHYTO2 MOI 0.1 N.D. N.D. N.D. -0.4 -0.3
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. N.D. -0.4 -0.3
phage 36hr PHYTO6 MOI 10 N.D. N.D. N.D. -0.5 -0.2
phage 36hr PHYTO2 MOI 1 N.D. N.D. N.D. -0.8 +0.1
phage 24hr PHYTO MOI 0.1 N.D. N.D. N.D. -0.5 +0.2
nitrogen source L-Isoleucine (N) N.D. N.D. N.D. +0.5 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. +0.6 +0.2
nitrogen source Carnitine Hydrochloride (N) N.D. N.D. N.D. +0.4 +0.4
carbon source D-Glucose 20 mM (C) N.D. N.D. N.D. +0.4 +0.4
carbon source Azelaic (C) N.D. N.D. N.D. +0.3 +0.4
carbon source Sebacic (C) N.D. N.D. N.D. +0.5 +0.3
carbon source Disodium Glutarate (C) N.D. N.D. N.D. +0.5 +0.3
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. N.D. N.D. +0.4 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. N.D. +0.3 +0.6
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