Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS16970 and BPHYT_RS16975 are separated by 48 nucleotidesBPHYT_RS16975 and BPHYT_RS16980 overlap by 4 nucleotidesBPHYT_RS16980 and BPHYT_RS16985 are separated by 206 nucleotidesBPHYT_RS16985 and BPHYT_RS16990 overlap by 4 nucleotides BPHYT_RS16970: BPHYT_RS16970 - monofunctional biosynthetic peptidoglycan transglycosylase, at 3,848,460 to 3,849,182 _RS16970 BPHYT_RS16975: BPHYT_RS16975 - shikimate 5-dehydrogenase, at 3,849,231 to 3,850,094 _RS16975 BPHYT_RS16980: BPHYT_RS16980 - ribonuclease II, at 3,850,091 to 3,852,208 _RS16980 BPHYT_RS16985: BPHYT_RS16985 - esterase, at 3,852,415 to 3,853,017 _RS16985 BPHYT_RS16990: BPHYT_RS16990 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase, at 3,853,014 to 3,854,408 _RS16990
Group Condition BPHYT_RS16970 BPHYT_RS16975 BPHYT_RS16980 BPHYT_RS16985 BPHYT_RS16990
carbon source D,L-Lactate (C) -0.0 -2.7 N.D. -0.8 -1.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.1 -2.1 N.D. -0.5 -2.2
nitrogen source L-Asparagine (N) +0.1 -3.4 N.D. -0.5 -0.7
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) -0.1 -1.6 N.D. -0.3 -2.4
nitrogen source L-Glutamic (N) -0.2 -2.7 N.D. -1.0 -0.3
carbon source D-Xylose (C) -0.0 -2.5 N.D. -1.2 -0.4
nitrogen source L-Glutamine (N) +0.2 -2.9 N.D. -0.5 -0.9
carbon source D,L-Lactate (C) -0.0 -2.8 N.D. -0.5 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days -0.0 -1.4 N.D. -0.5 -2.1
vitamin With vitamins t2 +0.2 -2.5 N.D. -0.7 -0.9
phage 36hr PHYTO10 MOI 10 -0.1 +0.2 N.D. -1.4 -2.3
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.0 -1.2 N.D. -0.5 -1.8
carbon source Xylitol (C) +0.0 -2.3 N.D. -0.8 -0.4
carbon source D-Galacturonic Acid (C) +0.1 -1.6 N.D. -0.3 -1.5
phage 36hr PHYTO10 MOI 10 -0.2 +0.4 N.D. -1.6 -1.9
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.0 -0.1 N.D. -0.8 -2.4
carbon source L-Fucose (C) +0.2 -2.3 N.D. -0.7 -0.5
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.0 -0.7 N.D. -0.9 -1.7
vitamin No vitamins t2 +0.1 -2.0 N.D. -0.3 -1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.5 -0.9 N.D. -0.1 -1.6
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.1 -1.1 N.D. -1.0 -1.1
nitrogen source L-Alanine (N) +0.1 -2.0 N.D. -0.7 -0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.1 -0.6 N.D. -0.4 -1.9
phage PHYTO3 MOI 0.1 -0.2 +0.1 N.D. -0.9 -2.1
nitrogen source L-Proline (N) +0.1 -1.8 N.D. -0.6 -0.7
carbon source L-Lactate (C) -0.0 -1.6 N.D. -0.3 -1.0
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.2 -0.7 N.D. -0.8 -1.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.7 N.D. -1.0 -0.9
stress Paraquat dichloride 0.064 mg/ml -0.0 -0.7 N.D. +1.1 +1.6
stress Cephalothin 2 mg/ml -0.1 +0.2 N.D. +0.1 +3.4
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