Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS16865 and BPHYT_RS16875 are separated by 873 nucleotidesBPHYT_RS16875 and BPHYT_RS16880 are separated by 83 nucleotidesBPHYT_RS16880 and BPHYT_RS16885 are separated by 231 nucleotidesBPHYT_RS16885 and BPHYT_RS16890 are separated by 112 nucleotides BPHYT_RS16865: BPHYT_RS16865 - ribonuclease, at 3,826,034 to 3,826,450 _RS16865 BPHYT_RS16875: BPHYT_RS16875 - integrase, at 3,827,324 to 3,828,124 _RS16875 BPHYT_RS16880: BPHYT_RS16880 - malic enzyme, at 3,828,208 to 3,830,547 _RS16880 BPHYT_RS16885: BPHYT_RS16885 - thiamine monophosphate kinase, at 3,830,779 to 3,831,777 _RS16885 BPHYT_RS16890: BPHYT_RS16890 - phosphatidylglycerophosphatase, at 3,831,890 to 3,832,348 _RS16890
Group Condition BPHYT_RS16865 BPHYT_RS16875 BPHYT_RS16880 BPHYT_RS16885 BPHYT_RS16890
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 +0.1 -5.2 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.1 -4.8 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.5 -0.1 -4.2 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.1 -0.1 -4.1 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.1 -0.0 -4.1 N.D. N.D.
carbon source L-Asparagine (C) -0.3 -0.0 -3.7 N.D. N.D.
carbon source Protocatechuic Acid (C) -0.5 -0.2 -3.1 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.3 +0.1 -3.4 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.4 -3.1 N.D. N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.1 +0.1 -3.6 N.D. N.D.
carbon source succinate (C) -0.1 -0.0 -2.9 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.7 -0.2 -1.9 N.D. N.D.
nitrogen source L-Asparagine (N) -0.4 +0.2 -2.6 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.4 +0.1 -2.3 N.D. N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.4 -0.4 -1.8 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.1 -2.7 N.D. N.D.
carbon source Suberic (C) -0.6 -0.1 -1.7 N.D. N.D.
tn7 efficiency Outgrowth after Tn7 transformation -0.1 +0.3 -2.5 N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.2 +0.1 -2.2 N.D. N.D.
nitrogen source L-Serine (N) -0.2 +0.2 -2.3 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.3 +0.3 -2.3 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 -0.0 -2.2 N.D. N.D.
carbon source succinate (C) +0.0 +0.2 -2.1 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.2 -1.9 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 -0.1 -1.9 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.6 +0.4 -1.3 N.D. N.D.
carbon source Phenylacetic 5 mM (C) -0.2 -0.4 +1.8 N.D. N.D.
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) +0.2 -0.1 +2.1 N.D. N.D.
nitrogen source L-Lysine (N) +0.6 +0.3 +1.8 N.D. N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.5 +0.2 +2.3 N.D. N.D.
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