Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS16705 and BPHYT_RS16710 are separated by 139 nucleotidesBPHYT_RS16710 and BPHYT_RS16715 are separated by 442 nucleotidesBPHYT_RS16715 and BPHYT_RS16720 are separated by 89 nucleotidesBPHYT_RS16720 and BPHYT_RS16725 are separated by 10 nucleotides BPHYT_RS16705: BPHYT_RS16705 - glutamate dehydrogenase, at 3,781,218 to 3,782,531 _RS16705 BPHYT_RS16710: BPHYT_RS16710 - LysR family transcriptional regulator, at 3,782,671 to 3,783,627 _RS16710 BPHYT_RS16715: BPHYT_RS16715 - adenylosuccinate lyase, at 3,784,070 to 3,785,458 _RS16715 BPHYT_RS16720: BPHYT_RS16720 - Gluconokinase (EC 2.7.1.12) (from data), at 3,785,548 to 3,786,045 _RS16720 BPHYT_RS16725: BPHYT_RS16725 - gluconate:H+ symporter (gntT) (from data), at 3,786,056 to 3,787,453 _RS16725
Group Condition BPHYT_RS16705 BPHYT_RS16710 BPHYT_RS16715 BPHYT_RS16720 BPHYT_RS16725
carbon source D-Gluconic Acid (C) N.D. -0.4 N.D. -5.6 -4.1
motility inner cut, LB soft agar motility assay N.D. -5.6 N.D. -0.3 -0.2
carbon source L-Arginine (C) N.D. -5.6 N.D. -0.1 -0.3
temperature LB, 23C N.D. -5.5 N.D. -0.0 -0.2
motility inner cut, LB soft agar motility assay N.D. -5.4 N.D. -0.2 -0.1
carbon source L-Histidine (C) N.D. -5.7 N.D. +0.1 -0.0
carbon source L-Proline (C) N.D. -5.6 N.D. -0.1 +0.0
motility outer cut, LB soft agar motility assay N.D. -5.5 N.D. +0.1 -0.2
motility outer cut, LB soft agar motility assay N.D. -5.4 N.D. -0.0 -0.2
carbon source L-Glutamine (C) N.D. -5.7 N.D. +0.0 +0.1
lb_dap plate control LB_DAP agar plate, outgrowth in LB N.D. -5.2 N.D. -0.4 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -4.9 N.D. -0.2 -0.2
nitrogen source L-Asparagine (N) N.D. -5.2 N.D. -0.3 +0.4
tn7 efficiency Outgrowth after Tn7 transformation N.D. -4.8 N.D. -0.3 -0.0
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. -4.9 N.D. -0.1 +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. -4.6 N.D. +0.4 -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -4.9 N.D. +0.2 -0.2
tn7 efficiency Outgrowth after Tn7 transformation N.D. -4.7 N.D. -0.3 +0.1
motility inner cut, LB soft agar motility assay N.D. -4.5 N.D. -0.2 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -4.0 N.D. -0.6 +0.0
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -4.5 N.D. +0.2 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB N.D. -4.8 N.D. +0.2 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -4.7 N.D. +0.3 +0.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. -4.5 N.D. -0.1 +0.4
supernatant control Vogels_fungal_media 0.1X N.D. -4.6 N.D. +0.1 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -4.8 N.D. +0.4 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -3.9 N.D. -0.4 +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. -4.6 N.D. +0.3 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -4.8 N.D. +0.6 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -4.9 N.D. +0.9 +0.7
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