Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS16530 and BPHYT_RS16535 are separated by 163 nucleotidesBPHYT_RS16535 and BPHYT_RS16540 are separated by 46 nucleotidesBPHYT_RS16540 and BPHYT_RS16545 are separated by 47 nucleotidesBPHYT_RS16545 and BPHYT_RS16550 are separated by 47 nucleotides BPHYT_RS16530: BPHYT_RS16530 - LysR family transcriptional regulator, at 3,746,745 to 3,747,728 _RS16530 BPHYT_RS16535: BPHYT_RS16535 - tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase, at 3,747,892 to 3,749,265 _RS16535 BPHYT_RS16540: BPHYT_RS16540 - phosphate starvation protein PhoH, at 3,749,312 to 3,750,385 _RS16540 BPHYT_RS16545: BPHYT_RS16545 - endoribonuclease YbeY, at 3,750,433 to 3,750,894 _RS16545 BPHYT_RS16550: BPHYT_RS16550 - calcium transporter ChaC, at 3,750,942 to 3,751,679 _RS16550
Group Condition BPHYT_RS16530 BPHYT_RS16535 BPHYT_RS16540 BPHYT_RS16545 BPHYT_RS16550
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.1 -0.6 -0.9 N.D. -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 -0.4 -0.3 N.D. -0.4
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.0 -0.7 -0.5 N.D. -0.4
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.1 -0.6 -0.6 N.D. -0.2
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.6 -0.3 +0.1 N.D. -0.4
phage 24hr PHYTO3 MOI 10 +0.5 -0.9 -0.0 N.D. -0.6
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.3 -0.6 -0.4 N.D. -0.2
phage 24hr PHYTO2 MOI 1 +0.6 -0.7 -0.5 N.D. -0.1
phage PHYTO3 MOI 0.1 +0.3 -0.8 -0.4 N.D. +0.3
phage 24hr PHYTO6 MOI 0.1 +0.4 -0.1 +0.2 N.D. -1.0
phage 24hr PHYTO3 MOI 10 +0.3 +0.3 -0.9 N.D. -0.1
phage P. phyto PHYTO2 MOI 0.1 +0.1 -0.3 +0.5 N.D. -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 -0.6 +0.2 N.D. -0.3
phage 36hr PHYTO2 MOI 1 +0.3 +0.0 +0.4 N.D. -0.9
nophagecontrol 24hr Phyto Lib Only +0.4 +0.2 +0.1 N.D. -0.9
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -0.2 +0.7 -0.4 N.D. -0.3
phage 36hr PHYTO8 MOI 10 +0.4 -0.5 +0.4 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 -0.1 -0.3 N.D. +0.8
phage 36hr PHYTO10 MOI 10 +0.4 -0.5 +0.4 N.D. -0.3
phage 24hr PHYTO3 MOI 1 +0.6 -0.3 +0.4 N.D. -0.7
phage 36hr PHYTO1 MOI 1 +0.2 -0.5 +0.6 N.D. -0.3
phage 36hr PHYTO4 MOI 1 +0.1 +0.5 +0.3 N.D. -0.6
phage 24hr PHYTO8 MOI 1 +0.5 -0.5 +0.4 N.D. -0.2
phage 36hr PHYTO7 MOI 1 +0.2 -0.6 +0.5 N.D. +0.1
phage 24hr PHYTO MOI 0.1 +0.3 +0.2 +0.5 N.D. -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 -0.6 +0.4 N.D. +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.8 -0.7 +0.3 N.D. +0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -0.5 +0.8 -0.0 N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.5 -0.4 +0.2 N.D. +0.4
phage 24hr PHYTO6 MOI 10 +0.1 +0.6 +0.6 N.D. -0.1
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