Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS16505 and BPHYT_RS16510 are separated by 245 nucleotidesBPHYT_RS16510 and BPHYT_RS16515 are separated by 450 nucleotidesBPHYT_RS16515 and BPHYT_RS16520 are separated by 188 nucleotidesBPHYT_RS16520 and BPHYT_RS16525 are separated by 16 nucleotides BPHYT_RS16505: BPHYT_RS16505 - HAD family hydrolase, at 3,742,072 to 3,742,758 _RS16505 BPHYT_RS16510: BPHYT_RS16510 - 3,4-Dihydroxy-2-butanone 4-phosphate synthase, at 3,743,004 to 3,743,723 _RS16510 BPHYT_RS16515: BPHYT_RS16515 - 2-hydroxyacid dehydrogenase, at 3,744,174 to 3,744,863 _RS16515 BPHYT_RS16520: BPHYT_RS16520 - major facilitator superfamily protein, at 3,745,052 to 3,746,218 _RS16520 BPHYT_RS16525: BPHYT_RS16525 - extradiol dioxygenase, at 3,746,235 to 3,746,633 _RS16525
Group Condition BPHYT_RS16505 BPHYT_RS16510 BPHYT_RS16515 BPHYT_RS16520 BPHYT_RS16525
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.1 -1.2 -0.5 -0.4 N.D.
phage 24hr PHYTO3 MOI 0.1 +0.1 +0.0 -1.5 -0.1 N.D.
phage 24hr PHYTO10 MOI 1 -0.0 +0.3 -1.2 -0.4 N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.0 -1.1 -0.3 +0.3 N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.2 -0.9 -0.4 +0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.6 -0.6 +0.5 +0.1 N.D.
carbon source D-Glucosamine Hydrochloride (C) -2.0 +0.7 +0.2 +0.3 N.D.
phage 36hr PHYTO2 MOI 1 +0.0 +0.2 -1.1 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.3 +0.6 -0.9 +0.1 N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -0.7 -0.3 +0.8 -0.1 N.D.
phage P. phyto PHYTO2 MOI 0.1 +0.4 +0.3 -1.1 +0.1 N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.9 -0.1 -0.2 +0.9 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.6 +0.7 +0.0 -0.3 N.D.
nophagecontrol 24hr Phyto Lib Only +0.3 +0.3 -1.1 +0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.4 -0.8 +0.6 +0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 +0.3 +0.6 -0.3 N.D.
nutrient casaminos -0.7 -0.2 +0.6 +0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 -0.3 -0.5 +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.7 -0.2 -0.6 +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.8 -0.6 +0.2 +0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.8 +0.4 -0.5 -0.0 N.D.
motility inner cut, LB soft agar motility assay +0.8 +0.7 -0.6 +0.3 N.D.
phage 36hr PHYTO11 MOI 0.1 +0.4 +0.1 +0.9 -0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days +1.0 +0.4 -0.1 +0.1 N.D.
phage 24hr PHYTO3 MOI 10 +1.0 +0.5 +0.1 -0.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days +0.5 +0.6 +0.4 +0.1 N.D.
carbon source Malonic (C) +0.5 +0.4 +0.7 +0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.8 +0.6 +0.7 +0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days +0.9 +0.6 +0.7 +0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days +0.7 +0.8 +1.1 +0.1 N.D.
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