Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS16215 and BPHYT_RS16220 are separated by 4 nucleotidesBPHYT_RS16220 and BPHYT_RS16225 are separated by 222 nucleotidesBPHYT_RS16225 and BPHYT_RS16230 are separated by 192 nucleotidesBPHYT_RS16230 and BPHYT_RS16235 are separated by 94 nucleotides BPHYT_RS16215: BPHYT_RS16215 - sensor histidine kinase, at 3,680,461 to 3,681,762 _RS16215 BPHYT_RS16220: BPHYT_RS16220 - transcriptional regulator, at 3,681,767 to 3,682,429 _RS16220 BPHYT_RS16225: BPHYT_RS16225 - D-alanyl-D-alanine carboxypeptidase, at 3,682,652 to 3,684,406 _RS16225 BPHYT_RS16230: BPHYT_RS16230 - C-5 sterol desaturase, at 3,684,599 to 3,685,561 _RS16230 BPHYT_RS16235: BPHYT_RS16235 - acylhydrolase, at 3,685,656 to 3,686,906 _RS16235
Group Condition BPHYT_RS16215 BPHYT_RS16220 BPHYT_RS16225 BPHYT_RS16230 BPHYT_RS16235
motility outer cut, LB soft agar motility assay -4.1 N.D. -0.4 -0.2 -0.1
motility outer cut, LB soft agar motility assay -3.8 N.D. -0.4 -0.1 -0.1
motility outer cut, LB soft agar motility assay -4.1 N.D. -0.3 -0.2 +0.1
motility inner cut, LB soft agar motility assay -2.9 N.D. -0.4 -0.1 +0.0
motility inner cut, LB soft agar motility assay -2.7 N.D. +0.0 -0.1 -0.3
motility inner cut, LB soft agar motility assay -2.6 N.D. -0.1 -0.1 -0.1
pH LB, pH4 -2.2 N.D. -0.4 -0.1 -0.1
stress Zinc 0.5 mM -3.0 N.D. -0.0 +0.1 +0.1
phage 36hr PHYTO1 MOI 1 -1.4 N.D. -0.7 -0.3 -0.1
pH LB, pH4 -2.1 N.D. -0.3 -0.0 -0.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -1.2 N.D. -0.8 -0.0 -0.4
nophagecontrol 24hr Phyto Lib Only -1.3 N.D. -0.6 -0.3 -0.2
stress Bacitracin 0.25 mg/ml -2.2 N.D. -0.0 -0.1 -0.0
stress Chloride 200 mM -1.0 N.D. -1.2 +0.0 -0.1
phage 36hr PHYTO11 MOI 1 -1.4 N.D. -0.7 -0.1 -0.1
phage 24hr PHYTO6 MOI 0.1 -1.1 N.D. -0.6 -0.4 -0.2
phage 24hr PHYTO3 MOI 1 -1.0 N.D. -0.8 -0.3 -0.1
phage 36hr PHYTO1 MOI 1 -1.0 N.D. -0.9 -0.3 -0.1
carbon source L-Arginine (C) -1.6 N.D. -0.3 -0.1 -0.1
phage 36hr PHYTO2 MOI 1 -0.8 N.D. -0.8 -0.2 -0.3
phage 24hr PHYTO MOI 0.1 -1.1 N.D. -0.6 -0.2 -0.1
phage 24hr PHYTO3 MOI 0.1 -0.8 N.D. -0.8 -0.4 -0.1
nophagecontrol 36hr Phyto Lib only -1.2 N.D. -0.6 -0.2 -0.1
stress Cephalothin 2 mg/ml -1.3 N.D. -0.5 -0.2 +0.0
phage 36hr PHYTO11 MOI 0.1 -1.0 N.D. -0.6 -0.3 -0.2
temperature LB 37C -1.9 N.D. -0.1 -0.1 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.6 N.D. +0.0 -0.3 -0.1
temperature LB, 37C -1.5 N.D. -0.1 -0.2 -0.2
stress A22 0.005 mg/ml -1.4 N.D. -0.3 -0.2 -0.1
phage 36hr PHYTO8 MOI 0.1 -0.8 N.D. -0.6 -0.4 -0.1
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