Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS16160 and BPHYT_RS16165 are separated by 168 nucleotidesBPHYT_RS16165 and BPHYT_RS16170 are separated by 1 nucleotidesBPHYT_RS16170 and BPHYT_RS16175 are separated by 18 nucleotidesBPHYT_RS16175 and BPHYT_RS16180 are separated by 428 nucleotides BPHYT_RS16160: BPHYT_RS16160 - purine permease, at 3,665,809 to 3,667,218 _RS16160 BPHYT_RS16165: BPHYT_RS16165 - multidrug transporter, at 3,667,387 to 3,668,964 _RS16165 BPHYT_RS16170: BPHYT_RS16170 - multidrug efflux RND transporter permease subunit, at 3,668,966 to 3,672,187 _RS16170 BPHYT_RS16175: BPHYT_RS16175 - hemolysin D, at 3,672,206 to 3,673,438 _RS16175 BPHYT_RS16180: BPHYT_RS16180 - TetR family transcriptional regulator, at 3,673,867 to 3,674,526 _RS16180
Group Condition BPHYT_RS16160 BPHYT_RS16165 BPHYT_RS16170 BPHYT_RS16175 BPHYT_RS16180
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.1 N.D. N.D. -1.8 -4.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.1 N.D. N.D. -3.0 -2.7
motility outer cut, LB soft agar motility assay -0.2 N.D. N.D. -3.9 -1.2
motility inner cut, LB soft agar motility assay -0.1 N.D. N.D. -4.3 -0.8
motility outer cut, LB soft agar motility assay -0.9 N.D. N.D. -4.1 +0.0
motility outer cut, LB soft agar motility assay +0.0 N.D. N.D. -4.5 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.4 N.D. N.D. -2.4 -2.5
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -0.4 N.D. N.D. -1.9 -2.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.4 N.D. N.D. -3.3 -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 N.D. N.D. -1.8 -2.6
supernatant Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.8X -0.3 N.D. N.D. -3.8 -0.0
motility inner cut, LB soft agar motility assay -0.4 N.D. N.D. -4.4 +0.9
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 N.D. N.D. -3.3 -0.9
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.4 N.D. N.D. -0.8 -3.3
supernatant control Vogels_fungal_media 0.1X +0.3 N.D. N.D. -4.5 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.3 N.D. N.D. -1.3 -2.4
stress Nickel (II) chloride 1 mM -0.2 N.D. N.D. -3.3 +0.5
carbon source Suberic (C) +0.0 N.D. N.D. +0.9 -3.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.9 N.D. N.D. -1.6 -2.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +1.0 N.D. N.D. -1.8 -2.0
stress Benzethonium chloride 0.0016 mM +0.1 N.D. N.D. -4.0 +1.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.1 N.D. N.D. +1.5 -3.6
phage 36hr PHYTO7 MOI 0.1 -0.6 N.D. N.D. +1.5 -2.1
stress Lomefloxacin 0.001 mM -0.2 N.D. N.D. -5.1 +4.5
phage 24hr PHYTO6 MOI 10 -0.4 N.D. N.D. +2.0 -1.7
phage 36hr PHYTO6 MOI 10 -0.5 N.D. N.D. +2.2 -1.6
phage P. phyto PHYTO2 MOI 1 -0.9 N.D. N.D. +1.4 +2.4
phage 24hr PHYTO7 MOI 1 -0.7 N.D. N.D. +1.3 +2.4
phage 24hr PHYTO3 MOI 10 -0.3 N.D. N.D. +1.0 +2.9
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.5 N.D. N.D. +1.7 +3.1
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