Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS16140 and BPHYT_RS16145 are separated by 58 nucleotidesBPHYT_RS16145 and BPHYT_RS16150 are separated by 14 nucleotidesBPHYT_RS16150 and BPHYT_RS16155 are separated by 230 nucleotidesBPHYT_RS16155 and BPHYT_RS16160 are separated by 426 nucleotides BPHYT_RS16140: BPHYT_RS16140 - ABC transporter permease, at 3,660,857 to 3,661,639 _RS16140 BPHYT_RS16145: BPHYT_RS16145 - S-formylglutathione hydrolase, at 3,661,698 to 3,662,549 _RS16145 BPHYT_RS16150: BPHYT_RS16150 - alcohol dehydrogenase, at 3,662,564 to 3,663,670 _RS16150 BPHYT_RS16155: BPHYT_RS16155 - carbohydrate kinase, at 3,663,901 to 3,665,382 _RS16155 BPHYT_RS16160: BPHYT_RS16160 - purine permease, at 3,665,809 to 3,667,218 _RS16160
Group Condition BPHYT_RS16140 BPHYT_RS16145 BPHYT_RS16150 BPHYT_RS16155 BPHYT_RS16160
phage 24hr PHYTO11 MOI 1 N.D. -0.2 -0.1 -0.1 -1.1
phage 36hr PHYTO4 MOI 1 N.D. -0.3 -0.3 +0.1 -1.0
phage 24hr PHYTO6 MOI 10 N.D. -0.3 -0.2 -0.5 -0.4
phage 24hr PHYTO9 MOI 0.1 N.D. -0.2 -0.2 -0.2 -0.8
phage P. phyto PHYTO2 MOI 1 N.D. -0.0 -0.1 -0.3 -0.9
motility outer cut, LB soft agar motility assay N.D. -0.3 +0.1 -0.3 -0.9
phage 24hr PHYTO MOI 0.1 N.D. -0.1 -0.1 -0.1 -1.0
phage 36hr PHYTO2 MOI 1 N.D. -0.4 -0.0 +0.4 -1.0
temperature LB, 37C N.D. -0.0 +0.2 -0.5 -0.6
phage 36hr PHYTO1 MOI 10 N.D. -0.4 -0.3 +0.3 -0.5
phage 24hr PHYTO6 MOI 0.1 N.D. -0.3 -0.1 +0.4 -0.8
phage 36hr PHYTO1 MOI 1 N.D. -0.2 -0.2 +0.2 -0.5
phage P. phyto PHYTO2 MOI 1 N.D. -0.4 -0.1 +0.5 -0.8
nitrogen source L-Lysine (N) N.D. +0.2 -0.1 -0.8 +0.2
phage 36hr PHYTO8 MOI 10 N.D. -0.3 +0.1 +0.3 -0.7
phage 36hr PHYTO11 MOI 0.1 N.D. +0.0 -0.1 +0.3 -0.8
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. +0.1 -0.3 +0.5 -0.8
nitrogen source L-Isoleucine (N) N.D. +0.3 -0.1 -0.7 +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.1 -0.1 +0.7 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.4 -0.2 +0.2 -0.4
carbon source Tween 20 (C) N.D. +0.2 -0.0 +0.4 -0.6
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. +0.3 -0.3 +0.5 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.8 +0.3 -0.2 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 -0.1 +0.4 +0.6
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. +0.1 +0.6 +0.5 -0.1
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. +0.2 +0.1 +0.4 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 +0.0 +0.5 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. +0.2 +0.3 +0.6 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 +0.1 +0.5 +0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 -0.0 +0.5 +1.0
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