Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS16105 and BPHYT_RS16110 overlap by 4 nucleotidesBPHYT_RS16110 and BPHYT_RS16115 are separated by 152 nucleotidesBPHYT_RS16115 and BPHYT_RS16120 are separated by 72 nucleotidesBPHYT_RS16120 and BPHYT_RS16125 are separated by 332 nucleotides BPHYT_RS16105: BPHYT_RS16105 - ABC transporter for D-Sorbitol, permease component 1 (from data), at 3,653,934 to 3,654,809 _RS16105 BPHYT_RS16110: BPHYT_RS16110 - ABC transporter for D-Sorbitol, permease component 2 (from data), at 3,654,806 to 3,655,741 _RS16110 BPHYT_RS16115: BPHYT_RS16115 - ABC transporter for D-Sorbitol, periplasmic substrate-binding component (from data), at 3,655,894 to 3,657,216 _RS16115 BPHYT_RS16120: BPHYT_RS16120 - sorbitol dehydrogenase, D-fructose forming (EC 1.1.1.14) (from data), at 3,657,289 to 3,658,071 _RS16120 BPHYT_RS16125: BPHYT_RS16125 - Fur family transcriptional regulator, at 3,658,404 to 3,658,898 _RS16125
Group Condition BPHYT_RS16105 BPHYT_RS16110 BPHYT_RS16115 BPHYT_RS16120 BPHYT_RS16125
carbon source D-Sorbitol (C) -3.5 -4.0 -3.8 -3.9 +0.2
motility outer cut, LB soft agar motility assay +0.1 -0.4 +0.2 -0.6 -0.3
nitrogen source L-Serine (N) +0.1 -0.7 +0.4 -0.1 -0.4
phage 24hr PHYTO6 MOI 1 -0.2 -1.0 +0.3 +0.4 -0.1
phage 36hr PHYTO9 MOI 1 -0.6 -0.3 +0.1 +0.5 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.1 -0.6 +0.7 +0.1 -0.5
phage 24hr PHYTO4 MOI 10 -0.3 -0.7 +0.3 +0.5 -0.2
phage 24hr PHYTO11 MOI 0.1 -0.3 -0.8 +0.1 +0.5 +0.1
phage 36hr PHYTO8 MOI 10 -0.1 -0.6 -0.2 +0.3 +0.3
phage 24hr PHYTO3 MOI 10 +0.2 -0.5 +0.3 -0.4 +0.2
phage 36hr PHYTO8 MOI 0.1 -0.3 -0.5 +0.2 +0.5 -0.0
carbon source D-Glucose (C) +0.3 +0.4 -0.2 -0.5 -0.1
phage 36hr PHYTO6 MOI 0.1 -0.1 -0.5 +0.3 +0.5 -0.3
phage 36hr PHYTO11 MOI 1 +0.1 -0.7 +0.3 +0.4 -0.1
phage 36hr PHYTO2 MOI 1 -0.3 -0.5 +0.1 +0.5 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.1 +0.2 +0.7 -0.2 -0.8
phage 24hr PHYTO7 MOI 1 -0.5 -0.4 -0.0 +0.6 +0.4
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.0 -0.5 +0.0 +0.9 -0.2
phage 24hr PHYTO11 MOI 1 -0.1 -0.6 +0.4 +0.5 -0.1
phage 36hr PHYTO3 MOI 1 -0.3 -0.4 +0.1 +0.6 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.6 +0.4 -0.3 -0.3 -0.0
phage 24hr PHYTO3 MOI 0.1 -0.1 -0.5 +0.2 +0.5 +0.3
phage 36hr PHYTO4 MOI 1 -0.1 -0.5 +0.1 +0.8 +0.2
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.2 -0.2 +0.1 +1.0 -0.6
phage 36hr PHYTO6 MOI 0.1 -0.1 -0.5 +0.2 +0.6 +0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.1 +0.3 +0.0 -0.5 +0.8
phage 36hr PHYTO6 MOI 1 -0.2 -0.4 +0.1 +0.7 +0.5
phage 36hr PHYTO4 MOI 1 -0.1 -0.3 +0.3 +0.8 +0.1
phage 36hr PHYTO6 MOI 10 -0.1 -0.0 +0.3 +0.6 +0.7
carbon source D-Xylose (C) +0.5 +0.5 +0.3 +0.1 +0.5
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