Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS16035 and BPHYT_RS16040 are separated by 852 nucleotidesBPHYT_RS16040 and BPHYT_RS16045 overlap by 4 nucleotidesBPHYT_RS16045 and BPHYT_RS16050 are separated by 46 nucleotidesBPHYT_RS16050 and BPHYT_RS16055 are separated by 37 nucleotides BPHYT_RS16035: BPHYT_RS16035 - tryptophan 2,3-dioxygenase, at 3,637,271 to 3,638,203 _RS16035 BPHYT_RS16040: BPHYT_RS16040 - short-chain dehydrogenase, at 3,639,056 to 3,639,793 _RS16040 BPHYT_RS16045: BPHYT_RS16045 - actin, at 3,639,790 to 3,641,334 _RS16045 BPHYT_RS16050: BPHYT_RS16050 - xylitol 2-dehydrogenase (EC 1.1.1.9) (from data), at 3,641,381 to 3,642,478 _RS16050 BPHYT_RS16055: BPHYT_RS16055 - sugar ABC transporter permease, at 3,642,516 to 3,643,523 _RS16055
Group Condition BPHYT_RS16035 BPHYT_RS16040 BPHYT_RS16045 BPHYT_RS16050 BPHYT_RS16055
carbon source Malonic (C) N.D. N.D. -0.4 -0.6 -0.7
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.7 -0.6 -0.4
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. N.D. -0.1 -0.6 -0.9
carbon source D-Glucose (C) N.D. N.D. -0.0 -0.4 -1.0
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days N.D. N.D. +0.1 -0.8 -0.4
phage 36hr PHYTO10 MOI 10 N.D. N.D. +0.2 -0.7 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.4 +0.2 -0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.2 -0.8 +0.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. N.D. -0.7 -0.8 +0.7
phage 36hr PHYTO11 MOI 0.1 N.D. N.D. +0.3 +0.2 -1.0
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. -0.2 -0.7 +0.4
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. N.D. +0.2 -0.9 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.2 -0.5 +0.6
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. N.D. -0.6 -0.0 +0.6
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. +0.5 +0.5 -1.0
carbon source Tween 20 (C) N.D. N.D. +0.4 +0.6 -0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. -0.2 +0.9 -0.4
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. -0.0 -0.5 +0.9
phage 36hr PHYTO4 MOI 1 N.D. N.D. +0.3 -0.4 +0.6
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. N.D. -0.3 +0.4 +0.6
phage 24hr PHYTO7 MOI 1 N.D. N.D. -0.3 +0.3 +0.7
phage 36hr PHYTO3 MOI 1 N.D. N.D. +0.4 -0.2 +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.1 -0.3 +1.4
supernatant control Vogels_fungal_media; pH 5.5 N.D. N.D. -0.2 +0.6 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.2 +0.4 +1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. N.D. +0.1 +0.5 +0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. +0.6 +0.7 +0.1
carbon source Xylitol (C) N.D. N.D. +3.8 -2.8 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +0.6 +0.6 +0.3
nitrogen source L-Glutamic (N) N.D. N.D. +0.6 +0.1 +1.1
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