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Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS16035 and BPHYT_RS16040 are separated by 852 nucleotides
BPHYT_RS16040 and BPHYT_RS16045 overlap by 4 nucleotides
BPHYT_RS16045 and BPHYT_RS16050 are separated by 46 nucleotides
BPHYT_RS16050 and BPHYT_RS16055 are separated by 37 nucleotides
BPHYT_RS16035: BPHYT_RS16035 - tryptophan 2,3-dioxygenase, at 3,637,271 to 3,638,203
_RS16035
BPHYT_RS16040: BPHYT_RS16040 - short-chain dehydrogenase, at 3,639,056 to 3,639,793
_RS16040
BPHYT_RS16045: BPHYT_RS16045 - actin, at 3,639,790 to 3,641,334
_RS16045
BPHYT_RS16050: BPHYT_RS16050 - xylitol 2-dehydrogenase (EC 1.1.1.9)
(from data)
, at 3,641,381 to 3,642,478
_RS16050
BPHYT_RS16055: BPHYT_RS16055 - sugar ABC transporter permease, at 3,642,516 to 3,643,523
_RS16055
Group
Condition
BPHYT
_RS16035
BPHYT
_RS16040
BPHYT
_RS16045
BPHYT
_RS16050
BPHYT
_RS16055
carbon source
Malonic (C)
N.D.
N.D.
-0.4
-0.6
-0.7
in planta
Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
-0.7
-0.6
-0.4
in planta
Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1
N.D.
N.D.
-0.1
-0.6
-0.9
carbon source
D-Glucose (C)
N.D.
N.D.
-0.0
-0.4
-1.0
in planta
Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days
N.D.
N.D.
+0.1
-0.8
-0.4
phage
36hr PHYTO10 MOI 10
N.D.
N.D.
+0.2
-0.7
-0.6
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
N.D.
-0.4
+0.2
-0.8
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
N.D.
-0.2
-0.8
+0.2
in planta
Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10
N.D.
N.D.
-0.7
-0.8
+0.7
phage
36hr PHYTO11 MOI 0.1
N.D.
N.D.
+0.3
+0.2
-1.0
replicable vector efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
N.D.
N.D.
-0.2
-0.7
+0.4
in planta
Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days
N.D.
N.D.
+0.2
-0.9
+0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
N.D.
-0.2
-0.5
+0.6
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
N.D.
-0.6
-0.0
+0.6
in planta
Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days
N.D.
N.D.
+0.5
+0.5
-1.0
carbon source
Tween 20 (C)
N.D.
N.D.
+0.4
+0.6
-0.7
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB
N.D.
N.D.
-0.2
+0.9
-0.4
replicable vector efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
N.D.
N.D.
-0.0
-0.5
+0.9
phage
36hr PHYTO4 MOI 1
N.D.
N.D.
+0.3
-0.4
+0.6
carbon source
2-Deoxyadenosine 5-monophosphate 5 mM (C)
N.D.
N.D.
-0.3
+0.4
+0.6
phage
24hr PHYTO7 MOI 1
N.D.
N.D.
-0.3
+0.3
+0.7
phage
36hr PHYTO3 MOI 1
N.D.
N.D.
+0.4
-0.2
+0.7
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
N.D.
-0.1
-0.3
+1.4
supernatant control
Vogels_fungal_media; pH 5.5
N.D.
N.D.
-0.2
+0.6
+0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
-0.2
+0.4
+1.0
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X
N.D.
N.D.
+0.1
+0.5
+0.7
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB
N.D.
N.D.
+0.6
+0.7
+0.1
carbon source
Xylitol (C)
N.D.
N.D.
+3.8
-2.8
+0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
N.D.
N.D.
+0.6
+0.6
+0.3
nitrogen source
L-Glutamic (N)
N.D.
N.D.
+0.6
+0.1
+1.1
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