Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS15970 and BPHYT_RS15975 are separated by 57 nucleotidesBPHYT_RS15975 and BPHYT_RS15980 are separated by 179 nucleotidesBPHYT_RS15980 and BPHYT_RS15985 are separated by 132 nucleotidesBPHYT_RS15985 and BPHYT_RS15990 are separated by 83 nucleotides BPHYT_RS15970: BPHYT_RS15970 - N-acetylmuramoyl-L-alanine amidase, at 3,624,073 to 3,625,653 _RS15970 BPHYT_RS15975: BPHYT_RS15975 - membrane protein, at 3,625,711 to 3,626,592 _RS15975 BPHYT_RS15980: BPHYT_RS15980 - pirin, at 3,626,772 to 3,627,647 _RS15980 BPHYT_RS15985: BPHYT_RS15985 - thioredoxin, at 3,627,780 to 3,628,628 _RS15985 BPHYT_RS15990: BPHYT_RS15990 - heme biosynthesis protein HemY, at 3,628,712 to 3,629,569 _RS15990
Group Condition BPHYT_RS15970 BPHYT_RS15975 BPHYT_RS15980 BPHYT_RS15985 BPHYT_RS15990
nitrogen source L-Lysine (N) N.D. +0.0 -0.2 -0.5 -0.8
phage 24hr PHYTO6 MOI 10 N.D. -0.0 -0.5 -0.1 -0.9
phage PHYTO3 MOI 0.1 N.D. -0.2 -0.6 +0.0 -0.5
stress A22 0.005 mg/ml N.D. -0.1 -0.3 -0.7 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.4 -0.5 +0.1 -0.4
phage 36hr PHYTO2 MOI 1 N.D. -0.6 +0.2 +0.2 -0.6
nitrogen source L-Serine (N) N.D. +0.1 -0.4 +0.3 -0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB N.D. +0.3 -0.4 -0.4 -0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.2 -0.1 +0.2 -0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB N.D. +0.4 -0.1 -0.6 -0.2
phage 36hr PHYTO3 MOI 1 N.D. -0.6 +0.3 +0.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.3 +0.2 -0.6 +0.2
phage 36hr PHYTO3 MOI 1 N.D. -0.8 +0.4 +0.2 -0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. -0.6 -0.0 -0.2 +0.6
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. +0.3 +0.1 -0.8 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.7 -0.1 -0.4 -0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.5 +0.1 +0.2 -0.7
nophagecontrol 24hr Phyto Lib only N.D. -0.4 -0.2 +0.4 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.6 +0.5 +0.2 +0.1
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. +1.3 -0.1 -0.5 -0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.0 -0.0 -0.4 +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.2 -0.1 +0.9 -0.1
phage 24hr PHYTO1 MOI 10 N.D. -0.0 +0.2 +0.7 -0.3
carbon source 2-Deoxy-D-Ribose (C) N.D. +0.5 +0.4 -0.3 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. +1.0 -0.0 +0.1 -0.3
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. +0.3 +0.4 -0.1 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. +0.6 -0.0 +0.4 +0.3
vitamin No vitamins t2 N.D. +0.1 +0.6 +0.1 +0.5
phage P. phyto corn MOI 1 N.D. +0.1 +0.6 +0.3 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.1 +0.5 +0.8 +0.1
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