Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS15605 and BPHYT_RS15610 are separated by 230 nucleotidesBPHYT_RS15610 and BPHYT_RS15615 are separated by 540 nucleotidesBPHYT_RS15615 and BPHYT_RS15620 are separated by 2 nucleotidesBPHYT_RS15620 and BPHYT_RS15625 are separated by 165 nucleotides BPHYT_RS15605: BPHYT_RS15605 - ABC transporter permease, at 3,539,596 to 3,540,546 _RS15605 BPHYT_RS15610: BPHYT_RS15610 - branched-chain amino acid ABC transporter substrate-binding protein, at 3,540,777 to 3,541,976 _RS15610 BPHYT_RS15615: BPHYT_RS15615 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 3,542,517 to 3,543,476 _RS15615 BPHYT_RS15620: BPHYT_RS15620 - peptidyl-prolyl cis-trans isomerase, at 3,543,479 to 3,543,934 _RS15620 BPHYT_RS15625: BPHYT_RS15625 - hypothetical protein, at 3,544,100 to 3,544,879 _RS15625
Group Condition BPHYT_RS15605 BPHYT_RS15610 BPHYT_RS15615 BPHYT_RS15620 BPHYT_RS15625
carbon source L-Proline (C) -5.0 -2.7 -1.0 -3.0 -0.2
nitrogen source L-Proline (N) -3.5 -2.6 -1.8 -2.6 +0.3
carbon source L-Glutamine (C) -2.2 -1.6 -1.6 -4.6 -0.0
carbon source L-Asparagine (C) -2.7 -2.2 -1.4 -1.7 -0.0
nitrogen source L-Lysine (N) -2.2 -0.8 -2.0 -2.5 -0.1
carbon source L-Tryptophan (C) -1.1 -0.5 -1.5 -3.7 -0.2
carbon source Tween 20 (C) -0.1 -0.4 -0.6 -6.0 +0.2
nitrogen source L-Glutamine (N) -0.2 -0.2 -2.0 -4.1 -0.0
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -1.6 -0.4 -1.8 -3.1 +0.4
carbon source Protocatechuic Acid (C) -0.2 +0.0 -1.4 -4.7 -0.0
pH LB, pH4 +0.1 +0.0 -2.0 -4.5 +0.1
carbon source L-Histidine (C) -1.2 -0.9 -1.1 -2.6 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.1 -1.6 -4.5 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.2 -0.1 -1.4 -4.1 -0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -0.3 -0.3 -1.4 -3.8 -0.0
carbon source L-Phenylalanine (C) -0.5 -0.3 -1.3 -3.7 +0.1
pH LB, pH4 +0.1 +0.1 -2.0 -4.1 +0.2
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.9 -0.2 -1.7 -2.4 -0.3
nitrogen source L-Phenylalanine (N) -1.5 -0.9 -0.3 -3.0 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -0.1 -0.7 -4.9 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.6 -0.1 -0.9 -3.7 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.3 +0.0 -1.1 -4.5 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.3 -0.0 -1.2 -3.7 -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.1 -0.3 -4.8 +0.3
motility inner cut, LB soft agar motility assay -0.1 +0.1 -2.2 -2.8 +0.2
carbon source Sebacic (C) +0.0 -0.1 +0.4 -4.8 +0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.1 -0.3 +1.1 -4.0 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 +0.0 -0.1 -4.6 +0.8
carbon source Disodium Glutarate (C) -0.2 -0.0 +0.3 +5.2 -0.0
carbon source Disodium Glutarate (C) -0.1 -0.2 +0.3 +5.4 +0.1
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