Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS15380 and BPHYT_RS15385 are separated by 15 nucleotidesBPHYT_RS15385 and BPHYT_RS15390 are separated by 201 nucleotidesBPHYT_RS15390 and BPHYT_RS15395 are separated by 49 nucleotidesBPHYT_RS15395 and BPHYT_RS15400 overlap by 4 nucleotides BPHYT_RS15380: BPHYT_RS15380 - diaminohydroxyphosphoribosylaminopyrimidine deaminase, at 3,491,753 to 3,492,904 _RS15380 BPHYT_RS15385: BPHYT_RS15385 - riboflavin synthase subunit alpha, at 3,492,920 to 3,493,534 _RS15385 BPHYT_RS15390: BPHYT_RS15390 - 3,4-Dihydroxy-2-butanone 4-phosphate synthase, at 3,493,736 to 3,494,872 _RS15390 BPHYT_RS15395: BPHYT_RS15395 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,494,922 to 3,495,428 _RS15395 BPHYT_RS15400: BPHYT_RS15400 - N utilization substance protein B homolog, at 3,495,425 to 3,495,859 _RS15400
Group Condition BPHYT_RS15380 BPHYT_RS15385 BPHYT_RS15390 BPHYT_RS15395 BPHYT_RS15400
phage 24hr PHYTO9 MOI 0.1 N.D. N.D. -0.8 N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. -0.7 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.7 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.7 N.D. N.D.
phage 24hr PHYTO3 MOI 10 N.D. N.D. -0.6 N.D. N.D.
motility outer cut, LB soft agar motility assay N.D. N.D. -0.6 N.D. N.D.
phage P. phyto PHYTO2 MOI 1 N.D. N.D. -0.5 N.D. N.D.
carbon source Azelaic (C) N.D. N.D. -0.5 N.D. N.D.
phage 24hr PHYTO3 MOI 10 N.D. N.D. -0.5 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.5 N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. -0.5 N.D. N.D.
carbon source Protocatechuic Acid (C) N.D. N.D. -0.5 N.D. N.D.
phage 36hr PHYTO6 MOI 1 N.D. N.D. -0.5 N.D. N.D.
phage 24hr PHYTO11 MOI 0.1 N.D. N.D. -0.5 N.D. N.D.
phage 24hr PHYTO8 MOI 1 N.D. N.D. -0.5 N.D. N.D.
carbon source D,L-Lactate (C) N.D. N.D. -0.5 N.D. N.D.
motility inner cut, LB soft agar motility assay N.D. N.D. -0.4 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.4 N.D. N.D.
carbon source D-Xylose (C) N.D. N.D. -0.4 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -0.4 N.D. N.D.
phage 24hr PHYTO10 MOI 10 N.D. N.D. -0.4 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.4 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. -0.4 N.D. N.D.
carbon source D,L-Lactate (C) N.D. N.D. -0.4 N.D. N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB N.D. N.D. -0.4 N.D. N.D.
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.4 N.D. N.D.
nophagecontrol 36hr Phyto Lib only N.D. N.D. +0.5 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. +0.5 N.D. N.D.
phage 36hr PHYTO8 MOI 10 N.D. N.D. +0.5 N.D. N.D.
stress Thallium(I) acetate 0.01 mg/ml N.D. N.D. +0.6 N.D. N.D.
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