Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS15335 and BPHYT_RS15340 are separated by 55 nucleotidesBPHYT_RS15340 and BPHYT_RS15345 are separated by 1501 nucleotidesBPHYT_RS15345 and BPHYT_RS15350 are separated by 851 nucleotidesBPHYT_RS15350 and BPHYT_RS15355 are separated by 192 nucleotides BPHYT_RS15335: BPHYT_RS15335 - coproporphyrinogen III oxidase, at 3,482,543 to 3,483,772 _RS15335 BPHYT_RS15340: BPHYT_RS15340 - tRNA-Ser, at 3,483,828 to 3,483,917 _RS15340 BPHYT_RS15345: BPHYT_RS15345 - hypothetical protein, at 3,485,419 to 3,485,727 _RS15345 BPHYT_RS15350: BPHYT_RS15350 - hypothetical protein, at 3,486,579 to 3,487,169 _RS15350 BPHYT_RS15355: BPHYT_RS15355 - endoribonuclease L-PSP, at 3,487,362 to 3,487,766 _RS15355
Group Condition BPHYT_RS15335 BPHYT_RS15340 BPHYT_RS15345 BPHYT_RS15350 BPHYT_RS15355
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.4 N.D. -0.8 -0.2 -0.4
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -1.0 N.D. -0.1 +0.6 -0.0
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.9 N.D. -0.1 +0.7 -0.0
phage P. phyto PHYTO2 MOI 1 +0.3 N.D. -0.3 +0.3 -0.6
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.8 N.D. +0.1 +0.3 +0.3
phage 36hr PHYTO4 MOI 0.1 +0.8 N.D. -0.4 -0.2 -0.2
phage 36hr PHYTO1 MOI 1 +0.7 N.D. -0.5 -0.3 +0.2
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.9 N.D. +0.3 +0.4 +0.4
phage 24hr PHYTO3 MOI 0.1 +1.0 N.D. -0.5 -0.3 +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 N.D. +0.2 +0.7 -0.3
phage 36hr PHYTO11 MOI 1 +0.8 N.D. -0.3 +0.2 -0.4
nophagecontrol 36hr Phyto Lib Only +0.8 N.D. -0.6 +0.2 -0.1
phage 24hr PHYTO4 MOI 0.1 +0.9 N.D. -0.4 -0.4 +0.2
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -0.5 N.D. +0.6 -0.1 +0.6
stress Chloride 200 mM +0.7 N.D. -0.3 +0.4 -0.2
phage 36hr PHYTO3 MOI 0.1 +1.0 N.D. -0.3 +0.2 -0.3
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.8 N.D. +0.3 +0.7 +0.4
nophagecontrol 24hr Phyto Lib Only +0.7 N.D. -0.4 +0.3 +0.2
phage 24hr PHYTO MOI 0.1 +0.9 N.D. -0.3 -0.0 +0.3
phage 36hr PHYTO3 MOI 1 +1.0 N.D. -0.2 -0.2 +0.3
phage 24hr PHYTO6 MOI 0.1 +0.9 N.D. -0.3 -0.0 +0.3
phage 36hr PHYTO10 MOI 1 +0.9 N.D. -0.3 +0.1 +0.3
phage 24hr PHYTO10 MOI 1 +0.9 N.D. -0.4 +0.1 +0.4
phage 24hr PHYTO3 MOI 10 +0.8 N.D. -0.2 -0.1 +0.5
phage 36hr PHYTO6 MOI 10 +0.9 N.D. -0.4 +0.1 +0.4
phage 24hr PHYTO2 MOI 0.1 +0.8 N.D. -0.2 +0.3 +0.2
phage 36hr PHYTO11 MOI 0.1 +0.8 N.D. -0.2 +0.2 +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 N.D. +0.1 +0.6 +0.9
phage 36hr PHYTO3 MOI 1 +1.0 N.D. -0.3 +0.0 +0.5
phage 24hr PHYTO3 MOI 1 +0.9 N.D. -0.1 +0.2 +0.4
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