Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS15270 and BPHYT_RS15275 are separated by 415 nucleotidesBPHYT_RS15275 and BPHYT_RS15280 are separated by 530 nucleotidesBPHYT_RS15280 and BPHYT_RS15285 are separated by 118 nucleotidesBPHYT_RS15285 and BPHYT_RS15290 are separated by 120 nucleotides BPHYT_RS15270: BPHYT_RS15270 - cold-shock protein, at 3,472,245 to 3,472,448 _RS15270 BPHYT_RS15275: BPHYT_RS15275 - hypothetical protein, at 3,472,864 to 3,473,112 _RS15275 BPHYT_RS15280: BPHYT_RS15280 - porin, at 3,473,643 to 3,474,788 _RS15280 BPHYT_RS15285: BPHYT_RS15285 - transcription elongation factor GreB, at 3,474,907 to 3,475,476 _RS15285 BPHYT_RS15290: BPHYT_RS15290 - tRNA-Arg, at 3,475,597 to 3,475,673 _RS15290
Group Condition BPHYT_RS15270 BPHYT_RS15275 BPHYT_RS15280 BPHYT_RS15285 BPHYT_RS15290
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. -0.6 +0.0 -1.0 -1.6
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.3 -0.3 -1.0 -1.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.4 -0.6 -1.1 -0.4
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. +0.1 -0.2 -2.2 -0.1
phage 36hr PHYTO10 MOI 10 N.D. +0.2 -0.9 -1.2 -0.4
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -0.3 -0.2 -2.0 +0.3
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. +0.6 -0.9 -0.6 -0.9
phage 36hr PHYTO8 MOI 10 N.D. -0.2 -0.4 -1.5 +0.3
phage 36hr PHYTO11 MOI 0.1 N.D. +0.3 -0.9 -1.2 +0.1
phage 24hr PHYTO2 MOI 0.1 N.D. +0.0 -0.5 -1.4 +0.4
carbon source Phenylacetic 5 mM (C) N.D. +0.7 -1.1 +0.2 -1.0
nitrogen source L-Lysine (N) N.D. -1.2 -0.5 +0.9 -0.4
phage 24hr PHYTO9 MOI 10 N.D. -0.6 -1.2 +0.0 +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.1 -0.6 -1.4 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.5 +0.3 -0.4 -1.4
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. +0.2 -0.7 +1.1 -1.5
phage 24hr PHYTO3 MOI 10 N.D. -0.0 +0.4 -2.1 +1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.8 +0.5 -1.5 -0.4
phage 36hr PHYTO6 MOI 0.1 N.D. +0.2 -1.3 +0.0 +0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.1 -0.4 -0.9 +1.0
phage 24hr PHYTO1 MOI 10 N.D. +0.1 -0.8 -0.6 +1.1
phage 24hr PHYTO1 MOI 10 N.D. -0.1 -1.4 +0.8 +0.7
stress Bacitracin 0.25 mg/ml N.D. +0.4 -1.0 -0.2 +0.8
phage 24hr PHYTO4 MOI 0.1 N.D. -0.4 -0.9 +0.8 +0.6
phage 36hr PHYTO6 MOI 0.1 N.D. +0.1 -1.3 +0.4 +1.0
phage 36hr PHYTO11 MOI 0.1 N.D. +0.2 -0.9 +0.6 +0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.5 -0.2 -0.4 +1.7
phage 36hr PHYTO8 MOI 10 N.D. +0.2 -1.1 +0.8 +1.2
nophagecontrol 36hr Phyto Lib only N.D. +0.4 -1.0 +1.0 +0.9
nophagecontrol 36hr Phyto Lib Only N.D. +0.1 -0.2 +1.4 +1.3
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BPHYT_RS15270
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BPHYT_RS15275
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BPHYT_RS15290
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