Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS15245 and BPHYT_RS15250 overlap by 4 nucleotidesBPHYT_RS15250 and BPHYT_RS15255 are separated by 590 nucleotidesBPHYT_RS15255 and BPHYT_RS15260 are separated by 272 nucleotidesBPHYT_RS15260 and BPHYT_RS15265 are separated by 129 nucleotides BPHYT_RS15245: BPHYT_RS15245 - transposase, at 3,467,867 to 3,468,703 _RS15245 BPHYT_RS15250: BPHYT_RS15250 - transposase, at 3,468,700 to 3,469,002 _RS15250 BPHYT_RS15255: BPHYT_RS15255 - hypothetical protein, at 3,469,593 to 3,469,742 _RS15255 BPHYT_RS15260: BPHYT_RS15260 - chorismate mutase, at 3,470,015 to 3,470,611 _RS15260 BPHYT_RS15265: BPHYT_RS15265 - DNA polymerase III subunit epsilon, at 3,470,741 to 3,471,892 _RS15265
Group Condition BPHYT_RS15245 BPHYT_RS15250 BPHYT_RS15255 BPHYT_RS15260 BPHYT_RS15265
phage 24hr PHYTO3 MOI 10 N.D. N.D. N.D. -1.4 -0.2
phage P. phyto corn MOI 1 N.D. N.D. N.D. -1.0 -0.3
phage P. phyto PHYTO2 MOI 1 N.D. N.D. N.D. -1.0 -0.3
phage 24hr PHYTO MOI 0.1 N.D. N.D. N.D. -0.9 -0.2
supernatant control Vogels_fungal_media 0.1X N.D. N.D. N.D. -0.9 -0.2
phage 36hr PHYTO2 MOI 1 N.D. N.D. N.D. -1.2 +0.1
phage 24hr PHYTO11 MOI 0.1 N.D. N.D. N.D. -1.0 -0.1
no phage control No phage control N.D. N.D. N.D. -0.7 -0.3
phage PHYTO3 MOI 0.1 N.D. N.D. N.D. -0.7 -0.3
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. -0.9 -0.1
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. N.D. N.D. -0.6 -0.4
phage P. phyto PHYTO2 MOI 0.1 N.D. N.D. N.D. -0.4 -0.5
temperature LB, 37C N.D. N.D. N.D. -0.8 -0.1
carbon source Tween 20 (C) N.D. N.D. N.D. -0.9 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. N.D. N.D. -0.8 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. -0.7 +0.2
phage 24hr PHYTO3 MOI 10 N.D. N.D. N.D. +0.2 -0.7
phage 36hr PHYTO1 MOI 1 N.D. N.D. N.D. +0.8 -0.8
phage 24hr PHYTO2 MOI 0.1 N.D. N.D. N.D. +0.5 -0.4
phage 36hr PHYTO6 MOI 10 N.D. N.D. N.D. +0.6 -0.3
phage 36hr PHYTO11 MOI 1 N.D. N.D. N.D. +0.8 -0.1
nitrogen source L-Alanine (N) N.D. N.D. N.D. +0.9 -0.1
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. N.D. N.D. +1.1 -0.3
phage 24hr PHYTO4 MOI 10 N.D. N.D. N.D. +1.0 -0.1
nitrogen source L-Lysine (N) N.D. N.D. N.D. +0.7 +0.3
nitrogen source Ammonium chloride (N) N.D. N.D. N.D. +0.8 +0.2
carbon source D-Glucose 20 mM (C) N.D. N.D. N.D. +1.0 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. N.D. +1.1 +0.1
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) N.D. N.D. N.D. +1.1 +0.1
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. N.D. N.D. +1.4 +0.0
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BPHYT_RS15250
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