Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS15235 and BPHYT_RS15240 are separated by 262 nucleotidesBPHYT_RS15240 and BPHYT_RS15245 are separated by 106 nucleotidesBPHYT_RS15245 and BPHYT_RS15250 overlap by 4 nucleotidesBPHYT_RS15250 and BPHYT_RS15255 are separated by 590 nucleotides BPHYT_RS15235: BPHYT_RS15235 - hypothetical protein, at 3,464,607 to 3,464,813 _RS15235 BPHYT_RS15240: BPHYT_RS15240 - excinuclease ABC subunit A, at 3,465,076 to 3,467,760 _RS15240 BPHYT_RS15245: BPHYT_RS15245 - transposase, at 3,467,867 to 3,468,703 _RS15245 BPHYT_RS15250: BPHYT_RS15250 - transposase, at 3,468,700 to 3,469,002 _RS15250 BPHYT_RS15255: BPHYT_RS15255 - hypothetical protein, at 3,469,593 to 3,469,742 _RS15255
Group Condition BPHYT_RS15235 BPHYT_RS15240 BPHYT_RS15245 BPHYT_RS15250 BPHYT_RS15255
supernatant control Vogels_fungal_media; pH 5.5 -0.8 -0.3 N.D. N.D. N.D.
phage 36hr PHYTO6 MOI 0.1 -0.7 -0.2 N.D. N.D. N.D.
phage 24hr PHYTO6 MOI 0.1 -0.7 -0.2 N.D. N.D. N.D.
phage 24hr PHYTO4 MOI 10 -0.8 -0.0 N.D. N.D. N.D.
phage 36hr PHYTO2 MOI 1 -0.8 -0.1 N.D. N.D. N.D.
phage 24hr PHYTO3 MOI 0.1 -0.8 -0.1 N.D. N.D. N.D.
phage 36hr PHYTO11 MOI 0.1 -0.7 -0.1 N.D. N.D. N.D.
nutrient L-Serine -0.7 -0.1 N.D. N.D. N.D.
phage 36hr PHYTO1 MOI 10 -0.8 -0.1 N.D. N.D. N.D.
phage 36hr PHYTO1 MOI 10 -0.8 -0.0 N.D. N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.8 +0.0 N.D. N.D. N.D.
phage 24hr PHYTO6 MOI 0.1 -0.7 -0.1 N.D. N.D. N.D.
phage P. phyto PHYTO2 MOI 1 -0.6 -0.1 N.D. N.D. N.D.
phage 36hr PHYTO10 MOI 1 -0.6 -0.1 N.D. N.D. N.D.
phage 36hr PHYTO10 MOI 10 -0.6 -0.1 N.D. N.D. N.D.
nophagecontrol 36hr Phyto Lib Only -0.5 -0.1 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.7 +0.1 N.D. N.D. N.D.
nitrogen source Ammonium chloride (N) -0.4 -0.2 N.D. N.D. N.D.
phage 36hr PHYTO2 MOI 1 -0.7 +0.0 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.8 +0.2 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.7 +0.2 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.6 +0.2 N.D. N.D. N.D.
nophagecontrol 24hr Phyto Lib only -0.5 +0.2 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.2 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.6 +0.3 N.D. N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.4 +0.3 N.D. N.D. N.D.
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.5 -0.1 N.D. N.D. N.D.
carbon source D-Glucose (C) +0.7 -0.1 N.D. N.D. N.D.
phage 24hr PHYTO9 MOI 10 +0.7 +0.0 N.D. N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.4 +0.3 N.D. N.D. N.D.
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