Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS15010 and BPHYT_RS15015 overlap by 4 nucleotidesBPHYT_RS15015 and BPHYT_RS15020 are separated by 137 nucleotidesBPHYT_RS15020 and BPHYT_RS15025 are separated by 113 nucleotidesBPHYT_RS15025 and BPHYT_RS15030 are separated by 302 nucleotides BPHYT_RS15010: BPHYT_RS15010 - cation transporter, at 3,413,416 to 3,414,516 _RS15010 BPHYT_RS15015: BPHYT_RS15015 - ArsR family transcriptional regulator, at 3,414,513 to 3,414,872 _RS15015 BPHYT_RS15020: BPHYT_RS15020 - tetratricopeptide repeat protein, at 3,415,010 to 3,416,857 _RS15020 BPHYT_RS15025: BPHYT_RS15025 - major facilitator transporter, at 3,416,971 to 3,418,272 _RS15025 BPHYT_RS15030: BPHYT_RS15030 - acetyltransferase, at 3,418,575 to 3,419,099 _RS15030
Group Condition BPHYT_RS15010 BPHYT_RS15015 BPHYT_RS15020 BPHYT_RS15025 BPHYT_RS15030
phage 24hr PHYTO2 MOI 1 -0.0 -1.6 -1.0 -0.2 -0.4
carbon source D-Glucose (C) -0.6 -3.0 -0.1 +0.2 +0.4
phage 36hr PHYTO3 MOI 1 -0.3 -1.5 -1.0 +0.1 -0.2
phage P. phyto PHYTO2 MOI 1 -0.2 -2.3 -0.6 +0.1 -0.1
phage 24hr PHYTO6 MOI 0.1 +0.0 -1.5 -0.7 -0.2 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 -2.6 -0.2 -0.3 +0.2
vitamin No vitamins t2 -0.4 -2.1 -0.2 -0.0 -0.0
phage 24hr PHYTO3 MOI 10 +0.3 -0.9 -0.8 -0.6 -0.5
motility outer cut, LB soft agar motility assay -0.2 -0.7 -1.5 +0.2 -0.4
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -0.3 -2.0 -0.4 +0.0 +0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -0.2 -1.9 -0.3 -0.2 +0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.2 -1.7 -0.6 -0.1 -0.2
phage 36hr PHYTO6 MOI 0.1 +0.2 -1.4 -0.7 +0.2 -0.6
stress Cobalt chloride 0.25 mM -4.4 +1.7 +0.3 -0.1 +0.1
carbon source 2-Deoxy-D-ribonic lithium salt (C) -0.2 -1.9 -0.2 -0.2 +0.2
carbon source Sebacic (C) +0.2 -2.5 +0.1 +0.1 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.9 -1.6 -0.1 -0.1 +0.8
phage 24hr PHYTO2 MOI 0.1 +0.6 -1.5 -0.6 -0.1 -0.3
nophagecontrol 36hr Phyto Lib only +0.4 -1.4 -0.7 -0.1 -0.0
carbon source adipate (C) +0.0 -2.0 -0.2 +0.1 +0.3
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.4 -1.6 -0.8 +0.1 +0.3
carbon source Suberic (C) +0.6 -1.7 -0.2 +0.3 +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.8 -1.1 -0.5 -0.2 +0.1
nophagecontrol 36hr Phyto Lib Only +0.0 +1.4 -0.4 -0.0 -0.8
supernatant control Vogels_fungal_media; pH 5.5 +0.8 +0.8 -0.3 -0.9 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 +1.6 +0.1 +0.0 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.9 +1.1 +0.3 -0.2 -0.2
carbon source D-Glucosamine Hydrochloride (C) -0.1 +2.3 -0.3 +0.1 -0.2
stress Nickel (II) chloride 1 mM -0.4 +2.8 -0.5 +0.0 -0.0
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X +0.9 +1.7 -0.2 +0.0 -0.0
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