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Protein
Homologs
Fitness for 5 genes in
Burkholderia phytofirmans PsJN
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Top 30 experiments (either direction), sorted by average fitness
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500 nt
BPHYT_RS15010 and BPHYT_RS15015 overlap by 4 nucleotides
BPHYT_RS15015 and BPHYT_RS15020 are separated by 137 nucleotides
BPHYT_RS15020 and BPHYT_RS15025 are separated by 113 nucleotides
BPHYT_RS15025 and BPHYT_RS15030 are separated by 302 nucleotides
BPHYT_RS15010: BPHYT_RS15010 - cation transporter, at 3,413,416 to 3,414,516
_RS15010
BPHYT_RS15015: BPHYT_RS15015 - ArsR family transcriptional regulator, at 3,414,513 to 3,414,872
_RS15015
BPHYT_RS15020: BPHYT_RS15020 - tetratricopeptide repeat protein, at 3,415,010 to 3,416,857
_RS15020
BPHYT_RS15025: BPHYT_RS15025 - major facilitator transporter, at 3,416,971 to 3,418,272
_RS15025
BPHYT_RS15030: BPHYT_RS15030 - acetyltransferase, at 3,418,575 to 3,419,099
_RS15030
Group
Condition
BPHYT
_RS15010
BPHYT
_RS15015
BPHYT
_RS15020
BPHYT
_RS15025
BPHYT
_RS15030
phage
24hr PHYTO2 MOI 1
-0.0
-1.6
-1.0
-0.2
-0.4
carbon source
D-Glucose (C)
-0.6
-3.0
-0.1
+0.2
+0.4
phage
36hr PHYTO3 MOI 1
-0.3
-1.5
-1.0
+0.1
-0.2
phage
P. phyto PHYTO2 MOI 1
-0.2
-2.3
-0.6
+0.1
-0.1
phage
24hr PHYTO6 MOI 0.1
+0.0
-1.5
-0.7
-0.2
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.0
-2.6
-0.2
-0.3
+0.2
vitamin
No vitamins t2
-0.4
-2.1
-0.2
-0.0
-0.0
phage
24hr PHYTO3 MOI 10
+0.3
-0.9
-0.8
-0.6
-0.5
motility
outer cut, LB soft agar motility assay
-0.2
-0.7
-1.5
+0.2
-0.4
tn7 efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50
-0.3
-2.0
-0.4
+0.0
+0.1
in planta
Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days
-0.2
-1.9
-0.3
-0.2
+0.1
in planta
Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days
+0.2
-1.7
-0.6
-0.1
-0.2
phage
36hr PHYTO6 MOI 0.1
+0.2
-1.4
-0.7
+0.2
-0.6
stress
Cobalt chloride 0.25 mM
-4.4
+1.7
+0.3
-0.1
+0.1
carbon source
2-Deoxy-D-ribonic lithium salt (C)
-0.2
-1.9
-0.2
-0.2
+0.2
carbon source
Sebacic (C)
+0.2
-2.5
+0.1
+0.1
-0.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
-0.9
-1.6
-0.1
-0.1
+0.8
phage
24hr PHYTO2 MOI 0.1
+0.6
-1.5
-0.6
-0.1
-0.3
nophagecontrol
36hr Phyto Lib only
+0.4
-1.4
-0.7
-0.1
-0.0
carbon source
adipate (C)
+0.0
-2.0
-0.2
+0.1
+0.3
replicable vector efficiency
Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50
+0.4
-1.6
-0.8
+0.1
+0.3
carbon source
Suberic (C)
+0.6
-1.7
-0.2
+0.3
+0.2
in planta
Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days
+0.8
-1.1
-0.5
-0.2
+0.1
nophagecontrol
36hr Phyto Lib Only
+0.0
+1.4
-0.4
-0.0
-0.8
supernatant control
Vogels_fungal_media; pH 5.5
+0.8
+0.8
-0.3
-0.9
-0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.6
+1.6
+0.1
+0.0
+0.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.9
+1.1
+0.3
-0.2
-0.2
carbon source
D-Glucosamine Hydrochloride (C)
-0.1
+2.3
-0.3
+0.1
-0.2
stress
Nickel (II) chloride 1 mM
-0.4
+2.8
-0.5
+0.0
-0.0
supernatant control:fungal media
R2A with Vogels_fungal_media 0.8X
+0.9
+1.7
-0.2
+0.0
-0.0
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