Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS14680 and BPHYT_RS14685 are separated by 7 nucleotidesBPHYT_RS14685 and BPHYT_RS14690 are separated by 101 nucleotidesBPHYT_RS14690 and BPHYT_RS14695 are separated by 24 nucleotidesBPHYT_RS14695 and BPHYT_RS14700 are separated by 235 nucleotides BPHYT_RS14680: BPHYT_RS14680 - peptidase C39, at 3,344,479 to 3,345,192 _RS14680 BPHYT_RS14685: BPHYT_RS14685 - peptidase C39, at 3,345,200 to 3,346,027 _RS14685 BPHYT_RS14690: BPHYT_RS14690 - hypothetical protein, at 3,346,129 to 3,347,565 _RS14690 BPHYT_RS14695: BPHYT_RS14695 - Fis family transcriptional regulator, at 3,347,590 to 3,349,158 _RS14695 BPHYT_RS14700: BPHYT_RS14700 - diacylglycerol kinase, at 3,349,394 to 3,349,894 _RS14700
Group Condition BPHYT_RS14680 BPHYT_RS14685 BPHYT_RS14690 BPHYT_RS14695 BPHYT_RS14700
stress Bacitracin 0.25 mg/ml -1.1 -0.8 -0.0 -0.0 N.D.
phage 36hr PHYTO10 MOI 10 -0.7 -0.6 -0.2 -0.2 N.D.
phage 24hr PHYTO6 MOI 10 -1.2 -0.2 -0.1 -0.0 N.D.
carbon source Tween 20 (C) +0.0 -1.2 +0.0 -0.4 N.D.
nophagecontrol 36hr Phyto Lib Only -1.0 +0.0 -0.2 -0.3 N.D.
phage 24hr PHYTO9 MOI 10 -0.2 -1.1 +0.0 -0.1 N.D.
carbon source Phenylacetic 5 mM (C) -0.7 -0.7 -0.1 +0.1 N.D.
phage 36hr PHYTO9 MOI 1 -0.2 -0.7 -0.1 -0.3 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB -0.4 -0.7 -0.3 +0.0 N.D.
phage 24hr PHYTO2 MOI 1 +0.2 -1.0 -0.2 -0.3 N.D.
phage 24hr PHYTO10 MOI 10 +0.2 -0.9 -0.3 -0.2 N.D.
phage 36hr PHYTO2 MOI 1 +0.2 -0.6 -0.2 -0.6 N.D.
phage 36hr PHYTO6 MOI 10 +0.1 -0.8 -0.3 -0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=15_days -0.3 -0.6 +0.2 -0.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.0 -0.1 -0.0 +0.2 N.D.
carbon source Suberic (C) -1.1 -0.2 +0.2 +0.1 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.5 -0.4 +0.3 -0.3 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.7 -0.3 +0.3 -0.2 N.D.
phage 36hr PHYTO2 MOI 0.1 +0.4 -0.8 -0.1 -0.3 N.D.
phage 36hr PHYTO8 MOI 10 +0.4 -0.7 -0.2 -0.1 N.D.
phage 24hr PHYTO4 MOI 10 +0.5 -0.8 -0.1 -0.1 N.D.
phage 24hr PHYTO11 MOI 1 +0.5 -0.8 +0.1 -0.2 N.D.
phage 24hr PHYTO6 MOI 1 +0.4 -0.9 +0.0 +0.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -0.7 +0.3 +0.2 N.D.
carbon source Suberic (C) -0.4 -0.4 +0.2 +0.3 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.6 -0.5 +0.2 -0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.7 +0.3 +0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.7 -0.4 +0.5 +0.2 N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 +0.5 -0.1 +0.3 +0.4 N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.5 +0.4 -0.1 +0.5 N.D.
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