Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS14355 and BPHYT_RS14360 overlap by 4 nucleotidesBPHYT_RS14360 and BPHYT_RS14365 overlap by 14 nucleotidesBPHYT_RS14365 and BPHYT_RS14370 are separated by 90 nucleotidesBPHYT_RS14370 and BPHYT_RS14375 are separated by 212 nucleotides BPHYT_RS14355: BPHYT_RS14355 - pyridoxine 5'-phosphate synthase, at 3,276,991 to 3,277,755 _RS14355 BPHYT_RS14360: BPHYT_RS14360 - DNA repair protein RecO, at 3,277,752 to 3,278,681 _RS14360 BPHYT_RS14365: BPHYT_RS14365 - GTPase Era, at 3,278,668 to 3,279,531 _RS14365 BPHYT_RS14370: BPHYT_RS14370 - ribonuclease III, at 3,279,622 to 3,280,851 _RS14370 BPHYT_RS14375: BPHYT_RS14375 - signal peptidase, at 3,281,064 to 3,281,957 _RS14375
Group Condition BPHYT_RS14355 BPHYT_RS14360 BPHYT_RS14365 BPHYT_RS14370 BPHYT_RS14375
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. -1.5 N.D. -0.8 N.D.
nitrogen source L-Phenylalanine (N) N.D. -0.2 N.D. -2.1 N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. -1.4 N.D. -0.8 N.D.
motility outer cut, LB soft agar motility assay N.D. -0.2 N.D. -1.9 N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. +0.0 N.D. -1.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.4 N.D. -1.4 N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. +0.1 N.D. -1.9 N.D.
temperature LB, 37C N.D. -0.3 N.D. -1.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. -0.6 N.D. -1.1 N.D.
carbon source Disodium Glutarate (C) N.D. -0.2 N.D. -1.5 N.D.
carbon source propionate (C) N.D. -0.4 N.D. -1.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.7 N.D. -0.8 N.D.
carbon source 2-Deoxy-D-Ribose (C) N.D. +0.1 N.D. -1.5 N.D.
carbon source D-Glucose 20 mM (C) N.D. -0.5 N.D. -0.9 N.D.
pH LB, pH4 N.D. -0.2 N.D. -1.2 N.D.
motility inner cut, LB soft agar motility assay N.D. -0.2 N.D. -1.2 N.D.
nutrient L-Serine N.D. -0.1 N.D. -1.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.7 N.D. -0.5 N.D.
stress Lomefloxacin 0.001 mM N.D. -0.3 N.D. -0.9 N.D.
carbon source D-Gluconic Acid (C) N.D. +0.2 N.D. -1.4 N.D.
motility outer cut, LB soft agar motility assay N.D. -0.1 N.D. -1.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. +0.2 N.D. -1.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.5 N.D. +0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. -0.3 N.D. +1.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 N.D. +1.1 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.3 N.D. +1.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. -0.4 N.D. +1.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.5 N.D. +1.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.0 N.D. +1.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.1 N.D. +1.9 N.D.
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