Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS14285 and BPHYT_RS14290 are separated by 88 nucleotidesBPHYT_RS14290 and BPHYT_RS14295 are separated by 191 nucleotidesBPHYT_RS14295 and BPHYT_RS14300 are separated by 727 nucleotidesBPHYT_RS14300 and BPHYT_RS14305 are separated by 105 nucleotides BPHYT_RS14285: BPHYT_RS14285 - drug:proton antiporter, at 3,263,947 to 3,264,441 _RS14285 BPHYT_RS14290: BPHYT_RS14290 - diguanylate cyclase, at 3,264,530 to 3,265,510 _RS14290 BPHYT_RS14295: BPHYT_RS14295 - GumN family protein, at 3,265,702 to 3,266,460 _RS14295 BPHYT_RS14300: BPHYT_RS14300 - tRNA-Cys, at 3,267,188 to 3,267,261 _RS14300 BPHYT_RS14305: BPHYT_RS14305 - tRNA-Gly, at 3,267,367 to 3,267,442 _RS14305
Group Condition BPHYT_RS14285 BPHYT_RS14290 BPHYT_RS14295 BPHYT_RS14300 BPHYT_RS14305
phage 36hr PHYTO2 MOI 0.1 -0.1 -0.3 -0.7 N.D. N.D.
nitrogen source L-Lysine (N) +0.1 -0.4 -0.5 N.D. N.D.
carbon source Phenylacetic 5 mM (C) +0.2 -0.4 -0.4 N.D. N.D.
carbon source Tween 20 (C) +0.5 -0.1 -0.7 N.D. N.D.
phage 24hr PHYTO2 MOI 1 -0.1 +0.4 -0.4 N.D. N.D.
phage 24hr PHYTO3 MOI 10 +0.1 +0.4 -0.6 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 +0.2 -0.5 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.2 +0.3 -0.4 N.D. N.D.
phage 36hr PHYTO3 MOI 1 +0.4 +0.3 -0.4 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.8 -0.3 N.D. N.D.
lb_nosalt LB_noSalt +0.3 -0.3 +0.3 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.6 +0.1 -0.3 N.D. N.D.
phage 36hr PHYTO4 MOI 1 +0.3 +0.5 -0.2 N.D. N.D.
stress Paraquat dichloride 0.032 mg/ml +0.3 -0.2 +0.5 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.6 +0.3 -0.2 N.D. N.D.
temperature LB, 37C -0.2 +0.2 +0.7 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.4 -0.2 +0.5 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 +1.1 -0.1 N.D. N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.2 -0.0 +0.7 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 +0.5 +0.2 N.D. N.D.
motility outer cut, LB soft agar motility assay +0.0 +0.2 +0.7 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.7 +0.1 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 +0.8 -0.1 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.7 +0.5 -0.2 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +0.3 +0.4 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.8 +0.0 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.8 +0.7 -0.4 N.D. N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.0 +0.4 +0.6 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.5 +0.3 +0.5 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 +0.6 +0.2 N.D. N.D.
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