Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS13980 and BPHYT_RS13985 are separated by 376 nucleotidesBPHYT_RS13985 and BPHYT_RS13990 are separated by 109 nucleotidesBPHYT_RS13990 and BPHYT_RS13995 are separated by 88 nucleotidesBPHYT_RS13995 and BPHYT_RS14000 are separated by 563 nucleotides BPHYT_RS13980: BPHYT_RS13980 - alanine--tRNA ligase, at 3,192,643 to 3,195,267 _RS13980 BPHYT_RS13985: BPHYT_RS13985 - CoA transferase, at 3,195,644 to 3,196,864 _RS13985 BPHYT_RS13990: BPHYT_RS13990 - hypothetical protein, at 3,196,974 to 3,197,534 _RS13990 BPHYT_RS13995: BPHYT_RS13995 - gamma-glutamyltranspeptidase, at 3,197,623 to 3,199,377 _RS13995 BPHYT_RS14000: BPHYT_RS14000 - glutaminyl-tRNA synthetase, at 3,199,941 to 3,201,650 _RS14000
Group Condition BPHYT_RS13980 BPHYT_RS13985 BPHYT_RS13990 BPHYT_RS13995 BPHYT_RS14000
carbon source Disodium Glutarate (C) N.D. -3.0 N.D. +0.3 N.D.
carbon source Disodium Glutarate (C) N.D. -2.8 N.D. +0.5 N.D.
phage 24hr PHYTO4 MOI 10 N.D. -0.5 N.D. -0.2 N.D.
phage 24hr PHYTO2 MOI 1 N.D. -0.6 N.D. -0.0 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.5 N.D. -0.1 N.D.
nitrogen source L-Lysine (N) N.D. -0.5 N.D. -0.1 N.D.
carbon source Tween 20 (C) N.D. -0.4 N.D. -0.1 N.D.
phage 24hr PHYTO6 MOI 1 N.D. -0.6 N.D. +0.0 N.D.
phage 24hr PHYTO MOI 0.1 N.D. -0.3 N.D. -0.2 N.D.
phage 36hr PHYTO7 MOI 1 N.D. -0.4 N.D. -0.1 N.D.
phage 24hr PHYTO7 MOI 1 N.D. -0.2 N.D. -0.3 N.D.
phage 24hr PHYTO11 MOI 0.1 N.D. -0.5 N.D. +0.2 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.5 N.D. +0.2 N.D.
phage 36hr PHYTO8 MOI 10 N.D. -0.4 N.D. +0.2 N.D.
supernatant control Vogels_fungal_media; pH 5.5 N.D. +0.3 N.D. -0.5 N.D.
nitrogen source L-Asparagine (N) N.D. +0.2 N.D. -0.3 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. +0.3 N.D. -0.3 N.D.
phage PHYTO3 MOI 0.1 N.D. +0.3 N.D. -0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.2 N.D. +0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.3 N.D. -0.2 N.D.
carbon source Suberic (C) N.D. +0.5 N.D. -0.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.3 N.D. +0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.3 N.D. +0.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.3 N.D. +0.2 N.D.
carbon source Suberic (C) N.D. +0.7 N.D. -0.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.3 N.D. +0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.2 N.D. +0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.2 N.D. +0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 N.D. +0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 N.D. +0.4 N.D.
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BPHYT_RS13980
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BPHYT_RS13985
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BPHYT_RS14000
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