Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS13820 and BPHYT_RS13825 are separated by 539 nucleotidesBPHYT_RS13825 and BPHYT_RS13830 are separated by 266 nucleotidesBPHYT_RS13830 and BPHYT_RS13835 are separated by 73 nucleotidesBPHYT_RS13835 and BPHYT_RS13840 overlap by 4 nucleotides BPHYT_RS13820: BPHYT_RS13820 - chemotaxis protein, at 3,151,089 to 3,152,762 _RS13820 BPHYT_RS13825: BPHYT_RS13825 - Fosmidomycin resistance protein, at 3,153,302 to 3,154,561 _RS13825 BPHYT_RS13830: BPHYT_RS13830 - acetyl-CoA synthetase, at 3,154,828 to 3,156,732 _RS13830 BPHYT_RS13835: BPHYT_RS13835 - S-adenosylhomocysteine nucleosidase, at 3,156,806 to 3,157,756 _RS13835 BPHYT_RS13840: BPHYT_RS13840 - DNA-dependent helicase, at 3,157,753 to 3,160,104 _RS13840
Group Condition BPHYT_RS13820 BPHYT_RS13825 BPHYT_RS13830 BPHYT_RS13835 BPHYT_RS13840
carbon source L-Alanine (C) -0.2 +0.0 -0.0 -2.3 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.3 -0.2 -0.0 -1.8 N.D.
carbon source Tween 20 (C) -0.4 -0.2 -0.0 -1.5 N.D.
phage 36hr PHYTO7 MOI 0.1 -0.6 -0.0 +0.1 -1.4 N.D.
nitrogen source L-Lysine (N) -0.4 -0.1 +0.0 -1.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 +0.4 +0.1 -2.3 N.D.
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -1.2 -0.0 +0.0 -0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -1.0 +0.1 -0.0 -0.9 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days +0.3 -0.0 -0.1 -2.0 N.D.
phage 36hr PHYTO7 MOI 1 -0.9 +0.1 +0.0 -0.9 N.D.
phage 24hr PHYTO MOI 0.1 +0.1 -0.1 +0.1 -1.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days -0.0 +0.2 +0.1 -1.7 N.D.
phage 24hr PHYTO4 MOI 0.1 -0.4 +0.1 +0.2 -1.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.0 +0.7 +0.1 -1.1 N.D.
motility outer cut, LB soft agar motility assay -0.3 +0.5 -0.1 -1.5 N.D.
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.7 -0.1 -0.0 -1.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 +0.8 +0.2 -1.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 +0.5 +0.2 -1.3 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.6 +0.1 -0.1 -1.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 +0.0 -0.1 -1.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.7 +1.0 +0.1 -0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.3 -0.1 +1.0 N.D.
nitrogen source Uridine (N) -0.3 -0.0 -0.0 +1.5 N.D.
phage 36hr PHYTO8 MOI 0.1 -0.1 -0.2 +0.3 +1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.3 -0.2 -0.0 +1.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.0 -0.2 +0.1 +1.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 +0.1 -0.1 +1.7 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 -0.1 -0.1 +1.7 N.D.
vitamin With vitamins t1 +0.1 +0.3 +0.0 +1.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 +0.2 +0.0 +1.8 N.D.
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