Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS13800 and BPHYT_RS13805 are separated by 300 nucleotidesBPHYT_RS13805 and BPHYT_RS13810 are separated by 31 nucleotidesBPHYT_RS13810 and BPHYT_RS13815 are separated by 1082 nucleotidesBPHYT_RS13815 and BPHYT_RS13820 are separated by 49 nucleotides BPHYT_RS13800: BPHYT_RS13800 - hypothetical protein, at 3,143,867 to 3,145,057 _RS13800 BPHYT_RS13805: BPHYT_RS13805 - type VI secretion protein ImpA, at 3,145,358 to 3,147,754 _RS13805 BPHYT_RS13810: BPHYT_RS13810 - type VI secretion protein, at 3,147,786 to 3,149,714 _RS13810 BPHYT_RS13815: BPHYT_RS13815 - hypothetical protein, at 3,150,797 to 3,151,039 _RS13815 BPHYT_RS13820: BPHYT_RS13820 - chemotaxis protein, at 3,151,089 to 3,152,762 _RS13820
Group Condition BPHYT_RS13800 BPHYT_RS13805 BPHYT_RS13810 BPHYT_RS13815 BPHYT_RS13820
phage 24hr PHYTO11 MOI 0.1 -0.4 -0.1 -0.7 -2.1 -0.0
phage 24hr PHYTO7 MOI 1 +0.2 -0.3 -0.6 -1.9 -0.3
phage 24hr PHYTO4 MOI 10 -0.3 -0.4 -0.7 -0.3 -1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.1 -0.8 -1.2 -0.3
phage 24hr PHYTO8 MOI 1 -0.1 -0.1 -0.3 -1.0 -0.6
phage PHYTO3 MOI 0.1 -0.4 -0.2 -0.7 -0.8 -0.2
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 -0.5 -0.2 -0.4 -0.7 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 -0.1 -0.8 -1.2 +0.1
phage 36hr PHYTO7 MOI 1 -0.7 -0.5 +0.4 -0.2 -0.9
phage 36hr PHYTO4 MOI 1 +0.2 -0.3 -0.5 -1.0 -0.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days +0.7 +0.0 -0.9 -0.1 -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.3 -0.1 -1.0 -1.0 +0.3
phage 24hr PHYTO4 MOI 0.1 -0.2 -0.3 +0.4 -0.9 -0.4
phage 36hr PHYTO8 MOI 10 +0.1 -0.1 +0.4 -1.1 -0.6
phage 36hr PHYTO8 MOI 10 -0.1 -0.4 +0.4 -0.9 -0.3
phage 24hr PHYTO10 MOI 1 +0.1 -0.3 +0.3 -0.8 -0.6
phage 36hr PHYTO2 MOI 1 -0.3 -0.4 +0.3 -0.8 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 +0.1 -1.7 -0.0 +0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.7 -0.0 -0.9 +0.7 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.3 -0.1 +0.6 +0.6 -1.0
phage 24hr PHYTO3 MOI 10 -0.5 +0.1 -0.6 +0.4 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.0 +0.0 -0.5 +1.0 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.1 +0.4 +0.7 -0.7
phage 36hr PHYTO11 MOI 1 -0.5 -0.4 +0.4 +1.0 -0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.4 +0.4 +0.7 -0.3 +0.3
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.2 +0.4 +0.7 -0.7 +0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +1.1 +0.2 -0.3 -0.1 +0.5
r2a control R2A control -0.1 +0.3 +0.7 +0.9 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.2 +0.2 +0.8 +0.5 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.1 -0.2 +1.1 +0.0 +0.9
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