Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS13775 and BPHYT_RS13780 overlap by 11 nucleotidesBPHYT_RS13780 and BPHYT_RS13785 are separated by 13 nucleotidesBPHYT_RS13785 and BPHYT_RS13790 are separated by 275 nucleotidesBPHYT_RS13790 and BPHYT_RS13795 overlap by 5 nucleotides BPHYT_RS13775: BPHYT_RS13775 - fusaric acid resistance protein, at 3,139,195 to 3,141,414 _RS13775 BPHYT_RS13780: BPHYT_RS13780 - hypothetical protein, at 3,141,404 to 3,141,604 _RS13780 BPHYT_RS13785: BPHYT_RS13785 - membrane protein, at 3,141,618 to 3,142,496 _RS13785 BPHYT_RS13790: BPHYT_RS13790 - hypothetical protein, at 3,142,772 to 3,142,960 _RS13790 BPHYT_RS13795: BPHYT_RS13795 - hypothetical protein, at 3,142,956 to 3,143,870 _RS13795
Group Condition BPHYT_RS13775 BPHYT_RS13780 BPHYT_RS13785 BPHYT_RS13790 BPHYT_RS13795
pH LB, pH4 -3.7 N.D. -5.3 N.D. -0.0
pH LB, pH4 -2.9 N.D. -4.9 N.D. -0.0
nitrogen source L-Lysine (N) -0.4 N.D. -0.6 N.D. -0.7
supernatant Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.8X -0.3 N.D. -0.7 N.D. -0.3
nitrogen source L-Serine (N) -0.2 N.D. -0.2 N.D. -0.8
supernatant Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.8X -0.4 N.D. -0.6 N.D. -0.2
supernatant Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.8X -0.3 N.D. -0.7 N.D. +0.1
phage 24hr PHYTO MOI 0.1 -0.3 N.D. +0.1 N.D. -0.6
phage 36hr PHYTO2 MOI 1 -0.3 N.D. +0.3 N.D. -0.5
phage 36hr PHYTO1 MOI 1 -0.3 N.D. +0.4 N.D. -0.5
motility outer cut, LB soft agar motility assay +0.4 N.D. -0.1 N.D. -0.6
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -0.4 N.D. -0.4 N.D. +0.5
phage 36hr PHYTO8 MOI 10 -0.3 N.D. -0.5 N.D. +0.5
phage 24hr PHYTO9 MOI 0.1 -0.2 N.D. -0.6 N.D. +0.6
carbon source Suberic (C) -0.2 N.D. +0.5 N.D. -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.3 N.D. +0.3 N.D. -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.0 N.D. -0.3 N.D. +0.8
phage 24hr PHYTO7 MOI 1 +0.1 N.D. +0.7 N.D. -0.2
phage 24hr PHYTO1 MOI 10 -0.3 N.D. +0.5 N.D. +0.4
supernatant control Vogels_fungal_media 0.1X +0.1 N.D. +0.6 N.D. +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.0 N.D. +0.3 N.D. +0.7
carbon source Phenylacetic 5 mM (C) +0.6 N.D. +0.5 N.D. -0.0
phage 36hr PHYTO10 MOI 1 +0.1 N.D. +0.5 N.D. +0.5
phage P. phyto PHYTO2 MOI 1 -0.0 N.D. +0.8 N.D. +0.4
supernatant control Vogels_fungal_media; pH 5.5 +0.1 N.D. +0.9 N.D. +0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 N.D. +0.8 N.D. +0.0
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.2 N.D. +0.3 N.D. +0.9
carbon source Phenylacetic 5 mM (C) +0.5 N.D. +0.4 N.D. +0.3
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.2 N.D. +0.6 N.D. +0.6
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 +0.2 N.D. +0.9 N.D. +0.2
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BPHYT_RS13775
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BPHYT_RS13780
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