Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS13495 and BPHYT_RS13500 overlap by 4 nucleotidesBPHYT_RS13500 and BPHYT_RS13505 overlap by 4 nucleotidesBPHYT_RS13505 and BPHYT_RS13510 are separated by 232 nucleotidesBPHYT_RS13510 and BPHYT_RS13515 are separated by 100 nucleotides BPHYT_RS13495: BPHYT_RS13495 - AsnC family transcriptional regulator, at 3,079,166 to 3,079,690 _RS13495 BPHYT_RS13500: BPHYT_RS13500 - AMP-dependent synthetase, at 3,079,687 to 3,081,348 _RS13500 BPHYT_RS13505: BPHYT_RS13505 - 2,4-dienoyl-CoA reductase, at 3,081,345 to 3,082,265 _RS13505 BPHYT_RS13510: BPHYT_RS13510 - C4-dicarboxylate transporter, at 3,082,498 to 3,083,745 _RS13510 BPHYT_RS13515: BPHYT_RS13515 - 3-isopropylmalate dehydratase small subunit, at 3,083,846 to 3,084,457 _RS13515
Group Condition BPHYT_RS13495 BPHYT_RS13500 BPHYT_RS13505 BPHYT_RS13510 BPHYT_RS13515
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.5 -1.2 -0.3 -0.6
supernatant control:fungal media R2A with Vogels_fungal_media 0.2X N.D. -0.1 -0.6 -0.1 -0.7
carbon source D-Glucose (C) N.D. +0.1 -0.7 -0.2 -0.6
phage 36hr PHYTO1 MOI 10 N.D. -0.1 +0.2 -0.0 -1.3
phage 36hr PHYTO1 MOI 1 N.D. -0.3 -0.4 +0.3 -0.6
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. -0.5 -1.2 +0.2 +0.6
phage 24hr PHYTO7 MOI 1 N.D. -0.2 -1.0 +0.1 +0.4
phage 24hr PHYTO9 MOI 0.1 N.D. -0.5 -0.7 +0.2 +0.3
phage 24hr PHYTO6 MOI 1 N.D. -0.1 -0.3 +0.4 -0.7
phage 36hr PHYTO1 MOI 10 N.D. -0.0 +0.6 +0.2 -1.3
phage 36hr PHYTO2 MOI 1 N.D. -0.1 +0.5 +0.2 -0.9
phage 24hr PHYTO4 MOI 0.1 N.D. -0.4 -0.5 +0.4 +0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.3 -0.4 -0.6 +0.4
phage P. phyto PHYTO2 MOI 1 N.D. +0.1 +0.4 +0.4 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.5 -0.2 -0.6 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. -0.2 -0.5 +0.6 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.3 -0.3 -0.3 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.0 +0.8 +0.4 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. +0.0 -0.5 +0.8 +0.3
carbon source Sebacic (C) N.D. -0.1 +0.7 -0.2 +0.5
carbon source Malonic (C) N.D. +0.2 +0.6 -0.2 +0.6
supernatant control Vogels_fungal_media 0.1X N.D. +0.5 -0.2 +0.1 +0.8
phage 36hr PHYTO8 MOI 10 N.D. -0.2 +1.2 +0.1 +0.2
supernatant control Vogels_fungal_media 0.1X N.D. +0.7 +0.4 -0.1 +0.5
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. +0.0 +0.5 -0.0 +0.9
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. +0.1 +0.5 -0.1 +0.9
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.6 +0.5 +0.4 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. +0.0 +0.5 -0.0 +1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. +0.0 +0.7 +0.1 +0.8
carbon source Suberic (C) N.D. +0.3 +1.0 +0.2 +0.1
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