Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS13475 and BPHYT_RS13480 are separated by 170 nucleotidesBPHYT_RS13480 and BPHYT_RS13485 are separated by 112 nucleotidesBPHYT_RS13485 and BPHYT_RS13490 are separated by 360 nucleotidesBPHYT_RS13490 and BPHYT_RS13495 are separated by 30 nucleotides BPHYT_RS13475: BPHYT_RS13475 - 2-keto-gluconate dehydrogenase, at 3,075,425 to 3,077,011 _RS13475 BPHYT_RS13480: BPHYT_RS13480 - hypothetical protein, at 3,077,182 to 3,077,853 _RS13480 BPHYT_RS13485: BPHYT_RS13485 - lactoylglutathione lyase, at 3,077,966 to 3,078,364 _RS13485 BPHYT_RS13490: BPHYT_RS13490 - hypothetical protein, at 3,078,725 to 3,079,135 _RS13490 BPHYT_RS13495: BPHYT_RS13495 - AsnC family transcriptional regulator, at 3,079,166 to 3,079,690 _RS13495
Group Condition BPHYT_RS13475 BPHYT_RS13480 BPHYT_RS13485 BPHYT_RS13490 BPHYT_RS13495
phage 24hr PHYTO2 MOI 1 -0.3 N.D. N.D. -1.5 N.D.
phage P. phyto PHYTO2 MOI 1 -0.8 N.D. N.D. -0.8 N.D.
phage 24hr PHYTO9 MOI 0.1 -0.6 N.D. N.D. -0.9 N.D.
phage 24hr PHYTO9 MOI 10 -0.6 N.D. N.D. -0.8 N.D.
phage 24hr PHYTO10 MOI 1 -0.1 N.D. N.D. -1.2 N.D.
phage 36hr PHYTO6 MOI 10 -0.3 N.D. N.D. -1.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 N.D. N.D. -0.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.7 N.D. N.D. -0.5 N.D.
phage 36hr PHYTO10 MOI 10 -0.1 N.D. N.D. -1.1 N.D.
phage 24hr PHYTO4 MOI 10 -0.3 N.D. N.D. -0.8 N.D.
phage 24hr PHYTO1 MOI 1 -0.2 N.D. N.D. -0.9 N.D.
phage 36hr PHYTO9 MOI 1 -0.7 N.D. N.D. -0.4 N.D.
nophagecontrol 24hr Phyto Lib Only -0.5 N.D. N.D. -0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.2 N.D. N.D. -1.0 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.4 N.D. N.D. +0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.8 N.D. N.D. -0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 N.D. N.D. +0.8 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.6 N.D. N.D. +0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X +0.6 N.D. N.D. +0.5 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days +0.4 N.D. N.D. +0.7 N.D.
supernatant control Vogels_fungal_media; pH 5.5 +0.3 N.D. N.D. +0.9 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days +0.4 N.D. N.D. +0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.4 N.D. N.D. -0.1 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.5 N.D. N.D. +0.9 N.D.
supernatant control Vogels_fungal_media 0.1X +0.1 N.D. N.D. +1.3 N.D.
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.4 N.D. N.D. +1.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.1 N.D. N.D. +1.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 N.D. N.D. +1.4 N.D.
in planta Plant=Brachypodium_distachyon ; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.8 N.D. N.D. +0.7 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB +0.5 N.D. N.D. +1.7 N.D.
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