Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS13420 and BPHYT_RS13425 overlap by 4 nucleotidesBPHYT_RS13425 and BPHYT_RS13430 are separated by 2 nucleotidesBPHYT_RS13430 and BPHYT_RS13435 overlap by 8 nucleotidesBPHYT_RS13435 and BPHYT_RS13440 are separated by 30 nucleotides BPHYT_RS13420: BPHYT_RS13420 - hypothetical protein, at 3,064,742 to 3,065,725 _RS13420 BPHYT_RS13425: BPHYT_RS13425 - histone acetyltransferase, at 3,065,722 to 3,066,333 _RS13425 BPHYT_RS13430: BPHYT_RS13430 - radical SAM protein, at 3,066,336 to 3,067,406 _RS13430 BPHYT_RS13435: BPHYT_RS13435 - aliphatic nitrilase, at 3,067,399 to 3,068,433 _RS13435 BPHYT_RS13440: BPHYT_RS13440 - hypothetical protein, at 3,068,464 to 3,068,946 _RS13440
Group Condition BPHYT_RS13420 BPHYT_RS13425 BPHYT_RS13430 BPHYT_RS13435 BPHYT_RS13440
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) +0.1 N.D. -1.0 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 N.D. -0.9 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 N.D. -1.3 N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent +0.5 N.D. -1.0 N.D. N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.9 N.D. +0.4 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.6 N.D. -0.9 N.D. N.D.
carbon source D-Glucose 20 mM (C) +0.4 N.D. -0.7 N.D. N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.8 N.D. +0.6 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.8 N.D. +0.6 N.D. N.D.
vitamin With vitamins t1 -0.6 N.D. +0.5 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.1 N.D. -1.2 N.D. N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.5 N.D. -0.6 N.D. N.D.
phage 24hr PHYTO7 MOI 10 +0.9 N.D. -0.8 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.6 N.D. -0.5 N.D. N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -0.6 N.D. +0.9 N.D. N.D.
motility inner cut, LB soft agar motility assay +0.8 N.D. -0.4 N.D. N.D.
carbon source 2-Deoxy-D-ribonic lithium salt (C) +1.1 N.D. -0.4 N.D. N.D.
carbon source D-Xylose (C) +0.7 N.D. +0.4 N.D. N.D.
phage 36hr PHYTO4 MOI 0.1 +0.6 N.D. +0.4 N.D. N.D.
carbon source Phenylacetic 5 mM (C) +0.8 N.D. +0.3 N.D. N.D.
temperature LB, 37C +0.7 N.D. +0.4 N.D. N.D.
phage 24hr PHYTO2 MOI 1 +0.2 N.D. +0.9 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.3 N.D. +0.8 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.0 N.D. +0.1 N.D. N.D.
nitrogen source L-Asparagine (N) +0.4 N.D. +0.9 N.D. N.D.
nitrogen source L-Lysine (N) +0.6 N.D. +0.7 N.D. N.D.
supernatant control Vogels_fungal_media 0.1X +0.9 N.D. +0.4 N.D. N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.9 N.D. +0.6 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +1.0 N.D. +1.2 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +2.2 N.D. +1.0 N.D. N.D.
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