Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS13140 and BPHYT_RS13145 are separated by 28 nucleotidesBPHYT_RS13145 and BPHYT_RS13150 are separated by 20 nucleotidesBPHYT_RS13150 and BPHYT_RS13155 are separated by 291 nucleotidesBPHYT_RS13155 and BPHYT_RS13160 are separated by 100 nucleotides BPHYT_RS13140: BPHYT_RS13140 - MFS transporter, at 3,001,263 to 3,002,462 _RS13140 BPHYT_RS13145: BPHYT_RS13145 - chemotaxis protein CheY, at 3,002,491 to 3,002,919 _RS13145 BPHYT_RS13150: BPHYT_RS13150 - hisitidine kinase, at 3,002,940 to 3,003,572 _RS13150 BPHYT_RS13155: BPHYT_RS13155 - bacterioferritin, at 3,003,864 to 3,004,436 _RS13155 BPHYT_RS13160: BPHYT_RS13160 - membrane protein, at 3,004,537 to 3,004,698 _RS13160
Group Condition BPHYT_RS13140 BPHYT_RS13145 BPHYT_RS13150 BPHYT_RS13155 BPHYT_RS13160
phage 36hr PHYTO4 MOI 1 -0.9 N.D. N.D. -0.5 -3.8
phage 24hr PHYTO MOI 0.1 -0.6 N.D. N.D. -0.5 -3.9
nophagecontrol 36hr Phyto Lib Only -0.4 N.D. N.D. -0.3 -4.0
nophagecontrol 24hr Phyto Lib Only +0.4 N.D. N.D. -0.1 -4.0
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -1.1 N.D. N.D. -0.3 -1.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 N.D. N.D. -0.1 -2.9
phage 36hr PHYTO8 MOI 10 -0.5 N.D. N.D. -0.2 -2.3
phage 24hr PHYTO2 MOI 1 -0.5 N.D. N.D. +0.0 -2.5
phage 36hr PHYTO10 MOI 10 -0.7 N.D. N.D. -0.8 -1.5
phage P. phyto corn MOI 1 -0.1 N.D. N.D. -0.4 -2.4
carbon source Deoxyribonucleic from herring sperm 10 mg/mL (C) -1.2 N.D. N.D. -0.1 -1.6
phage 24hr PHYTO6 MOI 0.1 -0.5 N.D. N.D. -0.7 -1.7
phage 24hr PHYTO4 MOI 10 -0.9 N.D. N.D. +0.1 -2.0
phage 36hr PHYTO6 MOI 1 +0.0 N.D. N.D. -0.3 -2.4
phage 24hr PHYTO11 MOI 0.1 -0.2 N.D. N.D. -0.3 -2.2
phage 24hr PHYTO6 MOI 1 -1.1 N.D. N.D. -0.3 -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 N.D. N.D. +0.1 -3.1
carbon source Phenylacetic 5 mM (C) -0.5 N.D. N.D. +0.1 -2.1
phage 36hr PHYTO6 MOI 0.1 +0.2 N.D. N.D. -0.1 -2.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.5 N.D. N.D. -0.4 -2.6
phage 36hr PHYTO1 MOI 1 -0.5 N.D. N.D. -0.3 -1.5
phage 36hr PHYTO1 MOI 10 -0.8 N.D. N.D. -0.2 -1.4
phage 36hr PHYTO4 MOI 0.1 -0.3 N.D. N.D. -0.7 -1.3
phage 24hr PHYTO3 MOI 1 -0.8 N.D. N.D. +0.2 -1.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.5 N.D. N.D. +0.0 -1.6
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.1 N.D. N.D. +0.5 -2.1
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days +0.2 N.D. N.D. +0.3 -1.8
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.8 N.D. N.D. -0.3 -1.7
vitamin With vitamins t2 +0.5 N.D. N.D. +0.3 -1.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.8 N.D. N.D. -0.4 +1.0
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