Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS13095 and BPHYT_RS13100 are separated by 459 nucleotidesBPHYT_RS13100 and BPHYT_RS13105 are separated by 182 nucleotidesBPHYT_RS13105 and BPHYT_RS13110 are separated by 153 nucleotidesBPHYT_RS13110 and BPHYT_RS13115 overlap by 4 nucleotides BPHYT_RS13095: BPHYT_RS13095 - hypothetical protein, at 2,993,385 to 2,993,831 _RS13095 BPHYT_RS13100: BPHYT_RS13100 - tRNA-Leu, at 2,994,291 to 2,994,375 _RS13100 BPHYT_RS13105: BPHYT_RS13105 - LacI family transcription regulator, at 2,994,558 to 2,995,628 _RS13105 BPHYT_RS13110: BPHYT_RS13110 - ABC transporter substrate-binding protein, at 2,995,782 to 2,996,870 _RS13110 BPHYT_RS13115: BPHYT_RS13115 - ABC transporter permease, at 2,996,867 to 2,997,691 _RS13115
Group Condition BPHYT_RS13095 BPHYT_RS13100 BPHYT_RS13105 BPHYT_RS13110 BPHYT_RS13115
carbon source propionate (C) N.D. N.D. -1.1 -0.3 +0.1
supernatant control Vogels_fungal_media; pH 5.5 N.D. N.D. -1.3 +0.2 +0.2
phage 36hr PHYTO9 MOI 0.1 N.D. N.D. -0.4 +0.7 -0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. -1.0 +0.4 +0.6
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.8 +0.7 +0.3
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. N.D. -0.8 +0.7 +0.4
phage 36hr PHYTO2 MOI 1 N.D. N.D. +0.4 +0.9 -0.7
phage P. phyto PHYTO2 MOI 1 N.D. N.D. +0.5 +0.9 -0.7
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.6 +0.6 +0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. -0.6 +0.6 +1.0
nophagecontrol 24hr Phyto Lib only N.D. N.D. +0.9 +0.5 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. +1.3 -0.3 +0.4
phage 24hr PHYTO4 MOI 0.1 N.D. N.D. +0.7 +0.8 -0.1
phage 36hr PHYTO8 MOI 10 N.D. N.D. +0.5 +0.5 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.6 -0.0 +0.9
phage 36hr PHYTO4 MOI 1 N.D. N.D. +0.3 +0.6 +0.7
phage 36hr PHYTO6 MOI 10 N.D. N.D. +0.7 +0.5 +0.5
phage 36hr PHYTO3 MOI 0.1 N.D. N.D. +0.7 +0.7 +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +1.1 +0.7 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. +1.2 +0.1 +0.3
phage 36hr PHYTO3 MOI 1 N.D. N.D. +0.6 +0.6 +0.4
phage 24hr PHYTO3 MOI 1 N.D. N.D. +0.8 +0.9 +0.0
phage 36hr PHYTO3 MOI 1 N.D. N.D. +0.4 +0.7 +0.6
phage 36hr PHYTO1 MOI 10 N.D. N.D. +0.8 +0.7 +0.3
phage 24hr PHYTO3 MOI 10 N.D. N.D. +0.9 +0.6 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. +1.5 -0.0 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.0 +0.3 +1.7
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. N.D. +0.9 +0.2 +1.1
phage 36hr PHYTO2 MOI 1 N.D. N.D. +0.6 +1.1 +0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. N.D. +2.2 -0.1 +0.3
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