Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS13065 and BPHYT_RS13070 are separated by 169 nucleotidesBPHYT_RS13070 and BPHYT_RS13075 are separated by 3 nucleotidesBPHYT_RS13075 and BPHYT_RS13080 are separated by 19 nucleotidesBPHYT_RS13080 and BPHYT_RS13085 are separated by 192 nucleotides BPHYT_RS13065: BPHYT_RS13065 - 2-aminoadipate aminotransferase, at 2,985,745 to 2,987,178 _RS13065 BPHYT_RS13070: BPHYT_RS13070 - oxidoreductase molybdopterin subunit, at 2,987,348 to 2,989,663 _RS13070 BPHYT_RS13075: BPHYT_RS13075 - hypothetical protein, at 2,989,667 to 2,990,170 _RS13075 BPHYT_RS13080: BPHYT_RS13080 - hypothetical protein, at 2,990,190 to 2,990,822 _RS13080 BPHYT_RS13085: BPHYT_RS13085 - hypothetical protein, at 2,991,015 to 2,991,323 _RS13085
Group Condition BPHYT_RS13065 BPHYT_RS13070 BPHYT_RS13075 BPHYT_RS13080 BPHYT_RS13085
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 +0.0 -0.0 -0.5 -3.8 N.D.
carbon source Phenylacetic 5 mM (C) +0.0 +0.2 -0.2 -3.8 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -0.0 -0.2 -0.3 -2.6 N.D.
nophagecontrol 24hr Phyto Lib Only -0.1 -0.0 -0.3 -2.4 N.D.
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 +0.2 -0.1 -0.0 -2.8 N.D.
phage 36hr PHYTO9 MOI 1 -0.3 -0.3 -0.4 -1.6 N.D.
phage 36hr PHYTO9 MOI 0.1 -0.0 +0.0 -0.1 -2.4 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB +0.1 -0.1 +0.3 -2.6 N.D.
phage 24hr PHYTO3 MOI 10 +0.0 +0.2 -0.1 -2.3 N.D.
phage 24hr PHYTO9 MOI 0.1 -0.2 +0.0 +0.0 -2.0 N.D.
nophagecontrol 24hr Phyto Lib only -0.1 +0.1 -0.0 -2.0 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.3 -0.3 -1.8 N.D.
stress Bacitracin 0.25 mg/ml +0.1 +0.1 -0.0 -2.2 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB -0.0 -0.1 -0.3 -1.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X +0.2 -0.0 +0.2 -2.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 -0.1 +0.1 -1.7 N.D.
phage 36hr PHYTO2 MOI 1 -0.1 +0.1 -0.6 -1.1 N.D.
carbon source Phenylacetic 5 mM (C) -0.1 +0.2 +0.0 -1.6 N.D.
carbon source D-Galacturonic Acid (C) +0.2 +0.1 -0.2 -1.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.1 +0.0 -1.6 N.D.
pH LB, pH4 -0.4 -0.8 +0.0 +0.6 N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 +0.1 +1.4 -1.6 N.D.
nophagecontrol 36hr Phyto Lib only +0.1 +0.4 -0.9 +0.6 N.D.
nitrogen source L-Lysine (N) -0.2 -0.0 -0.5 +1.3 N.D.
motility outer cut, LB soft agar motility assay -0.1 +0.0 -0.3 +1.4 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.2 -0.3 +1.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 +0.2 -0.2 +1.3 N.D.
phage P. phyto corn MOI 1 -0.2 +0.1 +0.1 +1.6 N.D.
motility inner cut, LB soft agar motility assay +0.1 -0.2 -0.1 +1.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.7 +0.2 -0.3 +1.8 N.D.
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