Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12865 and BPHYT_RS12870 are separated by 248 nucleotidesBPHYT_RS12870 and BPHYT_RS12875 are separated by 325 nucleotidesBPHYT_RS12875 and BPHYT_RS12880 are separated by 110 nucleotidesBPHYT_RS12880 and BPHYT_RS12885 are separated by 205 nucleotides BPHYT_RS12865: BPHYT_RS12865 - bifunctional protein FolD, at 2,934,716 to 2,935,576 _RS12865 BPHYT_RS12870: BPHYT_RS12870 - acetylase, at 2,935,825 to 2,936,940 _RS12870 BPHYT_RS12875: BPHYT_RS12875 - oligopeptidase A, at 2,937,266 to 2,939,374 _RS12875 BPHYT_RS12880: BPHYT_RS12880 - cytosine deaminase, at 2,939,485 to 2,940,777 _RS12880 BPHYT_RS12885: BPHYT_RS12885 - aspartate racemase, at 2,940,983 to 2,941,681 _RS12885
Group Condition BPHYT_RS12865 BPHYT_RS12870 BPHYT_RS12875 BPHYT_RS12880 BPHYT_RS12885
carbon source 2-Deoxy-D-ribonic lithium salt (C) N.D. +0.2 -1.6 +0.2 +0.1
carbon source 2-Deoxy-D-Ribose (C) N.D. -0.2 -0.6 -0.1 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. +0.1 +0.0 -0.4 -0.6
carbon source Phenylacetic 5 mM (C) N.D. +0.2 -0.8 +0.1 -0.2
phage 36hr PHYTO6 MOI 10 N.D. +0.1 -0.5 +0.2 -0.5
carbon source Phenylacetic 5 mM (C) N.D. +0.2 -0.8 +0.1 -0.1
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. -0.4 +0.0 +0.5 -0.6
phage 36hr PHYTO9 MOI 1 N.D. +0.4 -0.4 -0.0 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.3 -0.2 -0.1 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 -0.1 -0.3 -0.2
phage 24hr PHYTO3 MOI 10 N.D. +0.3 -0.1 +0.2 -0.6
phage 24hr PHYTO8 MOI 1 N.D. +0.2 +0.1 +0.2 -0.6
phage 24hr PHYTO10 MOI 10 N.D. +0.4 -0.4 +0.2 -0.2
phage 36hr PHYTO10 MOI 10 N.D. +0.4 -0.5 +0.1 -0.1
phage 36hr PHYTO6 MOI 1 N.D. +0.2 -0.2 +0.3 -0.4
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 N.D. +0.2 +0.3 +0.0 -0.5
phage P. phyto corn MOI 1 N.D. +0.5 -0.1 -0.2 -0.2
phage 24hr PHYTO9 MOI 10 N.D. +0.3 -0.4 +0.3 -0.2
phage 36hr PHYTO2 MOI 1 N.D. +0.5 -0.2 +0.1 -0.3
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. +0.4 -0.1 +0.3 -0.4
phage P. phyto PHYTO2 MOI 0.1 N.D. +0.1 +0.3 +0.3 -0.5
stress Nickel (II) chloride 1 mM N.D. +0.3 -0.4 +0.2 +0.1
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. -0.2 -0.2 +0.5 +0.2
phage 24hr PHYTO11 MOI 1 N.D. +0.6 -0.1 +0.3 -0.4
phage 24hr PHYTO4 MOI 10 N.D. +0.5 -0.3 +0.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.7 -0.3 +0.3 -0.1
carbon source 2-Deoxyadenosine 5-monophosphate 5 mM (C) N.D. +0.1 +0.9 -0.1 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.3 +0.3 +0.1 +0.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +0.3 -0.2 +0.6 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.2 +0.5 +0.3 +0.1
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