Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12770 and BPHYT_RS12775 are separated by 159 nucleotidesBPHYT_RS12775 and BPHYT_RS12780 are separated by 71 nucleotidesBPHYT_RS12780 and BPHYT_RS12785 are separated by 75 nucleotidesBPHYT_RS12785 and BPHYT_RS12790 are separated by 133 nucleotides BPHYT_RS12770: BPHYT_RS12770 - heme biosynthesis protein HemY, at 2,911,766 to 2,912,089 _RS12770 BPHYT_RS12775: BPHYT_RS12775 - FeS cluster assembly scaffold IscU, at 2,912,249 to 2,912,665 _RS12775 BPHYT_RS12780: BPHYT_RS12780 - cysteine desulfurase, at 2,912,737 to 2,913,960 _RS12780 BPHYT_RS12785: BPHYT_RS12785 - Rrf2 family transcriptional regulator, at 2,914,036 to 2,914,566 _RS12785 BPHYT_RS12790: BPHYT_RS12790 - phosphotyrosine protein phosphatase, at 2,914,700 to 2,915,197 _RS12790
Group Condition BPHYT_RS12770 BPHYT_RS12775 BPHYT_RS12780 BPHYT_RS12785 BPHYT_RS12790
stress Paraquat dichloride 0.032 mg/ml N.D. N.D. N.D. -4.1 N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. N.D. N.D. -3.5 N.D.
stress Paraquat dichloride 0.064 mg/ml N.D. N.D. N.D. -3.2 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. N.D. N.D. -2.8 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. N.D. N.D. -2.5 N.D.
pH LB, pH4 N.D. N.D. N.D. -2.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. -2.2 N.D.
stress Thallium(I) acetate 0.01 mg/ml N.D. N.D. N.D. -2.2 N.D.
phage 24hr PHYTO4 MOI 10 N.D. N.D. N.D. -2.0 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. N.D. -2.0 N.D.
phage 36hr PHYTO3 MOI 1 N.D. N.D. N.D. -2.0 N.D.
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. N.D. N.D. -1.9 N.D.
pH LB, pH4 N.D. N.D. N.D. -1.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. N.D. -1.9 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. -1.8 N.D.
phage 24hr PHYTO10 MOI 10 N.D. N.D. N.D. -1.7 N.D.
stress Cobalt chloride 0.25 mM N.D. N.D. N.D. -1.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. N.D. -1.7 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. N.D. N.D. -1.5 N.D.
phage 24hr PHYTO7 MOI 10 N.D. N.D. N.D. -1.5 N.D.
nitrogen source L-Asparagine (N) N.D. N.D. N.D. -1.5 N.D.
carbon source Phenylacetic 5 mM (C) N.D. N.D. N.D. -1.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI tmk3mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. N.D. N.D. -1.4 N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. N.D. N.D. -1.4 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. -1.4 N.D.
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. -1.4 N.D.
phage 36hr PHYTO1 MOI 1 N.D. N.D. N.D. -1.4 N.D.
carbon source Phenylacetic 5 mM (C) N.D. N.D. N.D. -1.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. +1.3 N.D.
phage 36hr PHYTO4 MOI 1 N.D. N.D. N.D. +1.7 N.D.
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