Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12700 and BPHYT_RS12705 are separated by 111 nucleotidesBPHYT_RS12705 and BPHYT_RS12710 are separated by 47 nucleotidesBPHYT_RS12710 and BPHYT_RS12715 overlap by 8 nucleotidesBPHYT_RS12715 and BPHYT_RS12720 overlap by 4 nucleotides BPHYT_RS12700: BPHYT_RS12700 - competence protein ComEC, at 2,895,170 to 2,897,959 _RS12700 BPHYT_RS12705: BPHYT_RS12705 - DNAase, at 2,898,071 to 2,898,859 _RS12705 BPHYT_RS12710: BPHYT_RS12710 - ABC transporter ATP-binding protein, at 2,898,907 to 2,899,650 _RS12710 BPHYT_RS12715: BPHYT_RS12715 - cell division protein FtsX, at 2,899,643 to 2,900,896 _RS12715 BPHYT_RS12720: BPHYT_RS12720 - hypothetical protein, at 2,900,893 to 2,901,090 _RS12720
Group Condition BPHYT_RS12700 BPHYT_RS12705 BPHYT_RS12710 BPHYT_RS12715 BPHYT_RS12720
phage 24hr PHYTO4 MOI 10 -0.7 -0.2 N.D. N.D. N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent -0.2 -0.5 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.1 -0.6 N.D. N.D. N.D.
phage 36hr PHYTO MOI 0.1 -0.6 -0.2 N.D. N.D. N.D.
phage 36hr PHYTO8 MOI 10 -0.3 -0.4 N.D. N.D. N.D.
phage 36hr PHYTO3 MOI 1 -0.4 -0.3 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.4 -0.2 N.D. N.D. N.D.
nophagecontrol 24hr Phyto Lib Only -0.5 +0.0 N.D. N.D. N.D.
nophagecontrol 36hr Phyto Lib Only -0.6 +0.2 N.D. N.D. N.D.
phage 36hr PHYTO6 MOI 0.1 -0.4 +0.2 N.D. N.D. N.D.
phage 36hr PHYTO3 MOI 1 -0.5 +0.4 N.D. N.D. N.D.
phage 24hr PHYTO6 MOI 0.1 -0.3 +0.2 N.D. N.D. N.D.
carbon source 2-Deoxy-D-Ribose (C) -0.3 +0.3 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -0.3 N.D. N.D. N.D.
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -0.4 +0.4 N.D. N.D. N.D.
phage 24hr PHYTO3 MOI 1 -0.2 +0.3 N.D. N.D. N.D.
phage 36hr PHYTO6 MOI 10 +0.3 -0.2 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB +0.4 -0.2 N.D. N.D. N.D.
phage P. phyto PHYTO2 MOI 1 -0.3 +0.6 N.D. N.D. N.D.
motility inner cut, LB soft agar motility assay -0.1 +0.4 N.D. N.D. N.D.
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -0.1 +0.5 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.6 N.D. N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.4 +0.2 N.D. N.D. N.D.
stress Nickel (II) chloride 1 mM +0.1 +0.5 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.3 +0.3 N.D. N.D. N.D.
nitrogen source L-Glutamine (N) +0.1 +0.5 N.D. N.D. N.D.
nitrogen source Putrescine (N) +0.2 +0.4 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 +0.5 N.D. N.D. N.D.
motility outer cut, LB soft agar motility assay -0.1 +0.8 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.7 N.D. N.D. N.D.
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