Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12655 and BPHYT_RS12660 are separated by 64 nucleotidesBPHYT_RS12660 and BPHYT_RS12665 are separated by 196 nucleotidesBPHYT_RS12665 and BPHYT_RS12670 are separated by 122 nucleotidesBPHYT_RS12670 and BPHYT_RS12675 are separated by 107 nucleotides BPHYT_RS12655: BPHYT_RS12655 - anhydrase, at 2,887,188 to 2,887,712 _RS12655 BPHYT_RS12660: BPHYT_RS12660 - Hsp33 chaperonin, at 2,887,777 to 2,888,727 _RS12660 BPHYT_RS12665: BPHYT_RS12665 - hypothetical protein, at 2,888,924 to 2,889,313 _RS12665 BPHYT_RS12670: BPHYT_RS12670 - cell division protein FtsB, at 2,889,436 to 2,889,873 _RS12670 BPHYT_RS12675: BPHYT_RS12675 - enolase, at 2,889,981 to 2,891,264 _RS12675
Group Condition BPHYT_RS12655 BPHYT_RS12660 BPHYT_RS12665 BPHYT_RS12670 BPHYT_RS12675
supernatant control Vogels_fungal_media; pH 5.5 N.D. -0.4 -1.4 -0.2 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. -0.0 -0.5 -1.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. -0.2 -0.6 -1.0 N.D.
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. -0.4 -0.2 -1.1 N.D.
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. -0.7 -0.6 -0.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. -0.3 -0.7 -0.5 N.D.
phage 24hr PHYTO6 MOI 1 N.D. -0.0 -0.3 -1.2 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.7 -0.5 -0.3 N.D.
phage 36hr PHYTO1 MOI 1 N.D. -0.1 +0.1 -1.5 N.D.
stress Cephalothin 2 mg/ml N.D. +0.1 -0.2 -1.1 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.6 -0.6 +0.4 N.D.
phage 24hr PHYTO6 MOI 10 N.D. +0.1 +0.4 -1.2 N.D.
no phage control No phage control N.D. -0.7 -0.5 +0.4 N.D.
phage 24hr PHYTO6 MOI 0.1 N.D. +0.0 +0.3 -1.1 N.D.
phage 36hr PHYTO7 MOI 0.1 N.D. -1.0 +0.4 +0.1 N.D.
motility outer cut, LB soft agar motility assay N.D. +0.7 -0.6 -0.5 N.D.
phage 36hr PHYTO10 MOI 10 N.D. -0.7 +0.5 -0.2 N.D.
phage P. phyto PHYTO2 MOI 1 N.D. -0.2 +0.6 -0.8 N.D.
phage 24hr PHYTO2 MOI 0.1 N.D. +0.1 +0.6 -1.1 N.D.
phage 36hr PHYTO11 MOI 0.1 N.D. -0.6 +0.7 +0.6 N.D.
vitamin No vitamins t2 N.D. -0.4 +0.7 +0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.4 +0.3 +0.7 N.D.
phage 24hr PHYTO8 MOI 1 N.D. -0.3 +0.5 +0.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.3 +1.7 -0.8 N.D.
carbon source Sebacic (C) N.D. +0.3 -0.0 +1.1 N.D.
carbon source Suberic (C) N.D. -0.0 +0.2 +1.3 N.D.
nitrogen source L-Lysine (N) N.D. +0.6 +0.5 +0.4 N.D.
phage 36hr PHYTO11 MOI 0.1 N.D. +0.2 +0.5 +0.9 N.D.
phage 36hr PHYTO8 MOI 10 N.D. +0.5 +0.4 +0.7 N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 N.D. +1.0 -0.1 +0.9 N.D.
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BPHYT_RS12655
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