Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12635 and BPHYT_RS12640 are separated by 130 nucleotidesBPHYT_RS12640 and BPHYT_RS12645 are separated by 119 nucleotidesBPHYT_RS12645 and BPHYT_RS12650 are separated by 83 nucleotidesBPHYT_RS12650 and BPHYT_RS12655 are separated by 784 nucleotides BPHYT_RS12635: BPHYT_RS12635 - translation factor Sua5, at 2,882,790 to 2,883,425 _RS12635 BPHYT_RS12640: BPHYT_RS12640 - phosphoesterase, at 2,883,556 to 2,884,386 _RS12640 BPHYT_RS12645: BPHYT_RS12645 - alpha/beta hydrolase, at 2,884,506 to 2,885,390 _RS12645 BPHYT_RS12650: BPHYT_RS12650 - hypothetical protein, at 2,885,474 to 2,886,403 _RS12650 BPHYT_RS12655: BPHYT_RS12655 - anhydrase, at 2,887,188 to 2,887,712 _RS12655
Group Condition BPHYT_RS12635 BPHYT_RS12640 BPHYT_RS12645 BPHYT_RS12650 BPHYT_RS12655
vitamin With vitamins t2 -0.5 -1.5 +0.2 +0.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -1.1 +0.2 -0.7 +0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 -0.6 -0.6 -0.5 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.5 -0.5 -0.7 -0.3 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB -1.6 +0.2 +0.4 +0.1 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.2X; growth supplemented with 0.4X LB -0.7 +0.4 +0.4 -0.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.0 -1.2 +0.2 +0.9 N.D.
in planta Plant=switchgrass; PlantTreatment=high_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=3weeks; NumberPlantsPooled=10 -0.3 +1.1 +0.1 -1.0 N.D.
phage 24hr PHYTO6 MOI 1 +1.0 +0.3 -0.6 -0.6 N.D.
phage 24hr PHYTO10 MOI 10 +1.0 +0.4 -0.1 -0.8 N.D.
phage 24hr PHYTO7 MOI 10 +0.9 +0.6 -0.5 -0.5 N.D.
phage PHYTO3 MOI 0.1 +1.4 -0.3 -0.0 -0.6 N.D.
phage 24hr PHYTO9 MOI 0.1 +0.7 +0.7 -0.6 -0.3 N.D.
vitamin With vitamins t1 +0.4 -0.8 +0.5 +0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.3 -0.6 +0.7 +0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.2 -0.9 +0.6 -0.3 N.D.
phage 24hr PHYTO MOI 0.1 +1.1 +0.5 -0.2 -0.7 N.D.
phage 36hr PHYTO8 MOI 10 +1.3 +0.1 -0.1 -0.6 N.D.
phage 36hr PHYTO9 MOI 0.1 +0.6 +0.5 +0.4 -0.6 N.D.
phage 36hr PHYTO8 MOI 10 +1.0 +0.8 -0.2 -0.6 N.D.
phage 36hr PHYTO6 MOI 1 +1.1 +0.6 -0.2 -0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 -0.4 +0.6 +0.9 N.D.
phage 24hr PHYTO6 MOI 0.1 +1.1 +0.9 -0.3 -0.3 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.4 -0.4 +0.3 +0.1 N.D.
phage 36hr PHYTO10 MOI 10 +0.9 +0.5 +0.7 -0.4 N.D.
phage 36hr PHYTO MOI 0.1 +1.3 +0.6 +0.3 -0.3 N.D.
nophagecontrol 36hr Phyto Lib only +1.2 +0.6 +0.2 -0.2 N.D.
phage 36hr PHYTO6 MOI 0.1 +1.0 +0.8 +0.1 +0.3 N.D.
phage 36hr PHYTO1 MOI 1 +1.2 +0.5 +0.3 +0.4 N.D.
phage 36hr PHYTO6 MOI 0.1 +1.0 +0.7 +0.5 +0.7 N.D.
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