Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12615 and BPHYT_RS12620 are separated by 43 nucleotidesBPHYT_RS12620 and BPHYT_RS12625 are separated by 35 nucleotidesBPHYT_RS12625 and BPHYT_RS12630 are separated by 6 nucleotidesBPHYT_RS12630 and BPHYT_RS12635 are separated by 47 nucleotides BPHYT_RS12615: BPHYT_RS12615 - dihydrodipicolinate synthase, at 2,879,263 to 2,880,189 _RS12615 BPHYT_RS12620: BPHYT_RS12620 - SAM-dependent methyltransferase PhcB, at 2,880,233 to 2,880,835 _RS12620 BPHYT_RS12625: BPHYT_RS12625 - tryptophanyl-tRNA synthetase, at 2,880,871 to 2,882,073 _RS12625 BPHYT_RS12630: BPHYT_RS12630 - peptidase M50, at 2,882,080 to 2,882,742 _RS12630 BPHYT_RS12635: BPHYT_RS12635 - translation factor Sua5, at 2,882,790 to 2,883,425 _RS12635
Group Condition BPHYT_RS12615 BPHYT_RS12620 BPHYT_RS12625 BPHYT_RS12630 BPHYT_RS12635
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -4.7 N.D. -0.2 N.D. -0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -4.5 N.D. +0.1 N.D. -1.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -5.0 N.D. +0.1 N.D. -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -5.7 N.D. +0.1 N.D. +0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -4.9 N.D. +0.3 N.D. -0.8
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -4.7 N.D. -0.2 N.D. -0.4
stress Bacitracin 0.25 mg/ml -5.7 N.D. +0.0 N.D. +0.3
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -4.6 N.D. -0.1 N.D. -0.4
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 -3.9 N.D. -0.6 N.D. -0.6
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -3.9 N.D. -0.2 N.D. -0.5
stress Benzethonium chloride 0.0016 mM -4.5 N.D. -0.0 N.D. -0.0
motility inner cut, LB soft agar motility assay -4.4 N.D. -0.2 N.D. +0.2
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days -4.0 N.D. -0.2 N.D. -0.2
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days -3.7 N.D. +0.1 N.D. -0.7
stress Bacitracin 0.125 mg/ml -3.8 N.D. +0.0 N.D. -0.3
motility inner cut, LB soft agar motility assay -4.0 N.D. -0.4 N.D. +0.4
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X -4.0 N.D. -0.1 N.D. +0.1
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days -3.1 N.D. +0.1 N.D. -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus -3.9 N.D. -0.0 N.D. +0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -3.8 N.D. -0.2 N.D. +0.2
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days -4.0 N.D. -0.4 N.D. +0.8
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -3.8 N.D. -0.0 N.D. +0.3
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days -3.7 N.D. +0.1 N.D. +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -3.7 N.D. +0.0 N.D. +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -3.4 N.D. -0.3 N.D. +0.5
tn7 efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pRH19; outgrowth in LB_gent50 -3.2 N.D. -0.2 N.D. +0.7
motility outer cut, LB soft agar motility assay -2.9 N.D. -0.3 N.D. +0.7
nitrogen source L-Lysine (N) -2.7 N.D. -0.2 N.D. +1.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -2.6 N.D. +0.0 N.D. +1.4
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -2.5 N.D. +0.4 N.D. +1.2
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