Fitness for 5 genes in Burkholderia phytofirmans PsJN

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500 ntBPHYT_RS12585 and BPHYT_RS12590 are separated by 217 nucleotidesBPHYT_RS12590 and BPHYT_RS12595 are separated by 161 nucleotidesBPHYT_RS12595 and BPHYT_RS12600 are separated by 305 nucleotidesBPHYT_RS12600 and BPHYT_RS12605 are separated by 130 nucleotides BPHYT_RS12585: BPHYT_RS12585 - XRE family transcriptional regulator, at 2,873,031 to 2,874,119 _RS12585 BPHYT_RS12590: BPHYT_RS12590 - dual-specificity RNA methyltransferase RlmN, at 2,874,337 to 2,875,488 _RS12590 BPHYT_RS12595: BPHYT_RS12595 - nucleoside diphosphate kinase, at 2,875,650 to 2,876,075 _RS12595 BPHYT_RS12600: BPHYT_RS12600 - membrane protein, at 2,876,381 to 2,877,079 _RS12600 BPHYT_RS12605: BPHYT_RS12605 - beta-lactamase, at 2,877,210 to 2,877,983 _RS12605
Group Condition BPHYT_RS12585 BPHYT_RS12590 BPHYT_RS12595 BPHYT_RS12600 BPHYT_RS12605
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth; Time=14_days N.D. -4.4 N.D. N.D. -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -3.7 N.D. N.D. -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -3.3 N.D. N.D. +0.1
in planta Plant=Arabidopsis_thaliana_col0; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_1.5%agar; Collection=outgrowth_LB; Time=7_days N.D. -2.9 N.D. N.D. -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X N.D. -3.1 N.D. N.D. +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -2.6 N.D. N.D. +0.0
in planta Plant=Zea_mays; PlantTreatment=NaCl_and_Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -2.2 N.D. N.D. -0.3
in planta Plant=Zea mays subsp. mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=14_days N.D. -2.0 N.D. N.D. -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=no_outgrowth_LB; Time=7_days N.D. -1.9 N.D. N.D. -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -1.9 N.D. N.D. -0.2
in planta Plant=Arabidopsis_thaliana_FLS2; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_0.8%agar; Collection=outgrowth_LB; Time=7_days N.D. -1.1 N.D. N.D. -1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB N.D. -2.5 N.D. N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.2 N.D. N.D. -0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -1.5 N.D. N.D. -0.3
carbon source Disodium Glutarate (C) N.D. -1.5 N.D. N.D. -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=root; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -1.4 N.D. N.D. -0.3
carbon source Suberic (C) N.D. -1.7 N.D. N.D. +0.1
in planta Plant=switchgrass; PreTreatment=None; PlantTreatment=low_phosphate; Sample=rhizosphere; GrowthSubstrate=Agar_0.5%; Collection=LB_with_kan50; Time=21days N.D. -1.8 N.D. N.D. +0.2
in planta Rhizosphere from sorghum RTx_430 plants, grown 2 months, low phosphate; Rep 1 N.D. -1.5 N.D. N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -1.5 N.D. N.D. +0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.2 N.D. N.D. +0.7
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -1.0 N.D. N.D. +0.9
replicable vector efficiency Conjugation on LB_DAP agar plate with WM3064 carrying pBBR1-MCS5; outgrowth in LB_gent50 N.D. +1.4 N.D. N.D. -0.6
phage 24hr PHYTO6 MOI 1 N.D. +1.4 N.D. N.D. -0.5
phage 36hr PHYTO8 MOI 0.1 N.D. +1.3 N.D. N.D. -0.3
phage 36hr PHYTO11 MOI 1 N.D. +1.6 N.D. N.D. -0.3
phage 36hr PHYTO11 MOI 0.1 N.D. +1.7 N.D. N.D. -0.2
phage 24hr PHYTO7 MOI 10 N.D. +1.7 N.D. N.D. +0.0
lb_dap plate control and outgrow on gent LB_DAP agar plate, outgrowth in LB_gent N.D. +1.8 N.D. N.D. -0.0
phage 36hr PHYTO7 MOI 1 N.D. +1.6 N.D. N.D. +0.5
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